Proximal genomic localization of STAT1 binding and regulated transcriptional activity

BMC Genomics

Table 2 Genes induced by IFN-γ in both human and mouse tissues, and associated STAT1 binding sites.

Gene Symbol	Description	STAT1 TFBS	Reference
BCL6	B-cell leukemia/lymphoma-6	+192 bp	[43]
CXCL10	Chemokine (C-X-C motif) ligand-10	-153 bp	[44]
CXCL9	Chemokine (C-X-C motif) ligand-9	-167 bp	[45-47]
GADD45γ	Growth arrest and DNA-damage-inducible 45 gamma	unknown
ID2	Inhibitor of DNA binding-2	unknown
IRF1	Interferon regulatory factor-1	-120 bp	[7]
IFIT2	Interferon-induced protein with tetratricopeptide repeats-2	-85 bp	[48]
SOCS1	Suppressor of cytokine signalling-1	unknown
SOCS3	Suppressor of cytokine signalling-3	-64 bp	[49]
TAP1	Transporter 1, ATP-binding cassette, sub-family B	+135 bp	[50]

Genes exhibiting significant (p < 0.01) differential expression in human microglial cells stimulated with IFN-γ for 1 h were identified by limma [34, 42] analysis of data associated with Rock et al.'s recent publication [17] (GEO id GSE1432). 80 orthologous mouse genes were identified using HCNetDat [51]. Differential expression levels in response to IFN-γ for the orthologous mouse genes were determined by affy [38-40] analysis of data associated with our recent publication of transcriptional responses to IFN-γ in IFN-γ^-/- mouse livers [19] (GEO id GSE4232). Literature was searched for experimentally verified STAT1 TFBSs (either GAS sites or ISRE sites were accepted) associated with the 10 most differentially regulated orthologous mouse genes (each was induced over 1.6-fold). Positions of STAT1 binding sites are given relative to the start of the first exon of each gene.

ISSN: 1471-2164