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Table 1 Strains and plasmids. Strains and plasmids used in this study, including their origin, reference and main characteristics

From: TasA-tasB, a new putative toxin-antitoxin (TA) system from Bacillus thuringiensis pGI1 plasmid is a widely distributed composite mazE-doc TA system

Strains or plasmids Relevant features Construction, source or reference
Strains   
   B. thuringiensis H1.1 Isolated from Ephestia kühniella, Canada IEBC (International Entomopathogenic Bacillus Center, Pasteur Institute, Paris, France)
   E. coli TOP10 Cloning host InVitrogen
   E. coli TG1 Cloning host Gibson, 1984
Plasmids   
   pGI1 Resident plasmid from B. thuringiensis H1.1 Mahillon and Seurinck, 1988
   pCR4-TOPO Positive-selection cloning vector, AmpR, KanR InVitrogen
   pCYB1 Cloning vector, AmpR, IPTG-inducible ptac promoter New England Biolabs Inc.
   pCYB10 pCYB1 deleted of intein gene J. Mahillon and F. Bilocq unpubl. results
   pBAD33 Cloning vector, CmR, arabinose-inducible promoter pBAD Guzman et al., 1995
   pGIF02 PCR fragment containing antitoxin gene of pGI1 cloned into pCR4-TOPO This study
   pGIF03 Nde I-Sal I pGIF02 fragment containing the antitoxin gene A cloned into pCYB1O This study
   pGIF04Mx PCR fragment containing the toxin gene P cloned into pCR4-TOPO, 4 clones obtained M1 → M4 This study
   pGIF06Mx PCR fragment containing the antitoxin-toxin locus AP cloned into pCR4-TOPO, 4 clones obtained M1 → M4 This study
   pGIF08M1 PCR fragment containing the antitoxin-toxin locus pAP cloned into pCR4-TOPO This study
   pGIF10Mx PCR fragment containing the antitoxin-toxin locus p2AP cloned into pCR4-TOPO, 15 clones obtained M1 → M15 This study
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