Figure 4
From: TILLING is an effective reverse genetics technique for Caenorhabditis elegans
Restriction enzyme digests of DNA from heterozygous and homozygous mutants. A) CAPS analysis of sibling lines for CN646 htp-3(vc1) using the restriction enzyme Taq1. The lanes labelled N2 are wildtype controls. Lane marked 4 exhibits additional bands when digested with this enzyme indicating this line is heterozygous for the vc1 mutation. B) CAPS analysis of sibling lines for CN711 mdf-2(vc15), using the restriction enzyme Hinf1. The lanes labelled N2 are wildtype controls. Lanes marked 4, 5 and 6 show additional cleavage bands and are missing the wildtype band indicating that they are homozygous for the vc15 mutation.