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Figure 5 | BMC Genomics

Figure 5

From: Novel design and controls for focused DNA microarrays: applications in quality assurance/control and normalization for the Health Canada ToxArray™

Figure 5

Comparison of effects of dilution of A. thaliana spike-in reference RNA in solution (target) and dilution of on-chip, spotted reference oligonucleotide (probe) in mouse cRNA hybridization reactions. Background-subtracted signal intensities are plotted against relative solution cRNA or attached oligonucleotide spot concentrations. Several murine genes with a range of expression levels were employed for slide-to-slide normalization of the spike-in RNA dilution series hybridization results.

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