Table 2 ToxArray™ data normalized without EC: Overall adjusted p after exposure to PB and fold changes (relative to Control) and pairwise p-values at each dose for each gene (probe) that showed differential expression. Bold text indicates genes identified as differential overall (p < 0.05) in the EC-normalized analysis (Table 1).
Control versus 100 mg/kg | Control versus 10 mg/kg | Control versus 1 mg/kg | James-Stein F-Test | ||||||
|---|---|---|---|---|---|---|---|---|---|
Gene (probe) Name | Full name | GenBank | Fold change | Pairwise P-value | Fold change | Pairwise P-value | Fold change | Pairwise P-value | Overall P-value |
Cyp2b9* | cytochrome P450, 2b9, phenobarbitol inducible, type a, | NM_010000 | 8.55 | 0.0000 | 3.56 | 0.0000 | 1.15 | 0.8412 | 0.0000 |
Cyp2b10 | Cytochrome P450, 2b10, phenobarbitol inducible, type b | NM_009998 | 3.93 | 0.0000 | 2.92 | 0.0000 | -1.08 | 0.8501 | 0.0000 |
Gdf15 | growth differentiation factor 15 | NM_011819 | 2.57 | 0.0000 | 1.26 | 0.6381 | 1.11 | 0.8311 | 0.0002 |
Gsta2 | Glutathione S-transferase, alpha 2 (Yc2) | NM_008182 | 2.26 | 0.0353 | 1.08 | 0.9165 | -1.22 | 0.7337 | 0.0160 |
Gstm3 | glutathione S- transferase mu 3 (EC 2.5.1.18) | J03953 | 2.21 | 0.0000 | -1.01 | 0.9793 | 1.31 | 0.4323 | 0.0002 |
Gadd45b | growth arrest and DNA-damage-inducible 45 beta | NM_008655 | 1.93 | 0.0000 | 1.04 | 0.8931 | -1.12 | 0.6016 | 0.0000 |
Lpin1 | lipin 1, alias fatty liver dystrophy | NM_015763 | -1.66 | 0.0037 | -1.02 | 0.9469 | 1.03 | 0.9303 | 0.0051 |
Gadd45a | growth arrest and DNA damage inducible | NM_007836 | 1.62 | 0.0024 | 1.25 | 0.4642 | -1.22 | 0.4506 | 0.0000 |
Idi1 | isopentenyl-diphosphate delta isomerase | NM_145360 | -1.57 | 0.0131 | -1.44 | 0.2098 | -1.04 | 0.9206 | 0.0160 |
Por | NADPH cytochrome p450 oxidoreductase | NM_008898 | 1.55 | 0.0489 | 1.34 | 0.4157 | 1.07 | 0.8477 | 0.0341 |
Rbp1 | retinol binding protein 1, cellular | NM_011254 | 1.49 | 0.0029 | 1.04 | 0.8850 | 1.14 | 0.5150 | 0.0094 |
AhR | rat Ah receptor | NM_013464 | 1.42 | 0.0676 | 1.06 | 0.8713 | -1.07 | 0.8304 | 0.0436 |
Dusp6 | dual specificity phosphatase 6 | NM_026268 | 1.41 | 0.0486 | 1.29 | 0.3460 | -1.04 | 0.9018 | 0.0107 |
Gsta4 | Glutathione S-transferase, alpha 4 | NM_010357 | 1.41 | 0.0037 | -1.00 | 0.9961 | -1.09 | 0.6389 | 0.0006 |
gstm2 | glutathione S-transferase, mu 2 | NM_008183 | 1.40 | 0.0131 | 1.05 | 0.8594 | 1.07 | 0.7447 | 0.0329 |
Ephx1 | epoxide hydrolase 1, microsomal; Eph1, Eph-1, AI195553 | NM_010145 | 1.39 | 0.0004 | 1.08 | 0.6873 | 1.11 | 0.4882 | 0.0021 |
Chk | choline kinase | NM_013490 | -1.39 | 0.0214 | -1.17 | 0.5202 | 1.03 | 0.9165 | 0.0223 |
Ugt1a6 | UDP glycosyltransferase 1 family, polypeptide A6 | XM_135531 | 1.38 | 0.0019 | 1.22 | 0.2979 | 1.13 | 0.4624 | 0.0063 |
Lbp | Lipopolysaccharide binding protein | NM_008489 | 1.38 | 0.0254 | 1.12 | 0.6547 | 1.35 | 0.1677 | 0.0436 |
Adcy6 | adenylate cyclase 6 | NM_007405 | -1.34 | 0.0037 | -1.04 | 0.8511 | 1.01 | 0.9774 | 0.0068 |
Pitpn | phosphatidylinositol transfer protein | NM_008850 | 1.23 | 0.0000 | 1.07 | 0.4947 | 1.08 | 0.4311 | 0.0004 |
Olfr447 | olfactory receptor 447 | NM_146988 | -1.20 | 0.6328 | -1.19 | 0.4947 | 1.03 | 0.9112 | 0.0436 |
Anpep | aminopeptidase N | NM_008486 | 1.20 | 0.3851 | -1.14 | 0.5202 | -1.02 | 0.9253 | 0.0223 |
cd8b | CD8 antigen, beta chain | NM_009858 | -1.16 | 0.0158 | -1.07 | 0.5160 | -1.11 | 0.2616 | 0.0436 |
Cyp2a4 | Cytochrome P450, 2a4 (phenobarbitol inducer | NM_009997 | 1.14 | 0.3851 | -1.07 | 0.6474 | -1.09 | 0.4999 | 0.0045 |
Mmp9: | matrix metalloproteinase 9 | NM_013599 | -1.14 | 0.0735 | -1.15 | 0.1425 | -1.16 | 0.1632 | 0.0305 |
Egfr | epidermal growth factor receptor | NM_007912 | -1.13 | 0.9847 | 1.07 | 0.8206 | 1.30 | 0.1971 | 0.0192 |
Hao1 | hydroxyacid oxidase | NM_010403 | -1.12 | 0.9867 | 1.24 | 0.5202 | 1.42 | 0.1991 | 0.0233 |
Gpam | glycerol-3-phosphate acyltransferase, mitochondrial | NM_008149 | -1.10 | 0.9847 | 1.11 | 0.5202 | 1.20 | 0.1971 | 0.0063 |
Ape2 | AP endonuclease 2 | NM_029943 | -1.09 | 0.9867 | -1.18 | 0.5833 | -1.43 | 0.1677 | 0.0395 |
Acadvl | acyl-Coenzyme A dehydrogenase, very long chain | NM_017366 | -1.07 | 0.9867 | 1.10 | 0.5887 | 1.18 | 0.2294 | 0.0436 |
SCADfamilyprotein | mRNA for SCAD family protein | AJ296079 | -1.05 | 0.9890 | 1.02 | 0.9388 | 1.26 | 0.1971 | 0.0395 |
PERP | PERP, TP53 apoptosis effector | NM_022032 | -1.04 | 0.9890 | 1.06 | 0.7161 | 1.15 | 0.2892 | 0.0170 |
Acaa2 | acetyl-Coenzyme A acyltransferase 2, mitochondrial | NM_177470 | -1.02 | 0.9984 | 1.11 | 0.5202 | 1.24 | 0.1632 | 0.0094 |
Vegfb(2) | vascular endothelial growth factor B | NM_011697 | -1.02 | 0.9984 | -1.25 | 0.2731 | -1.31 | 0.1677 | 0.0101 |
Fads2 | fatty acid desaturase 2 | NM_019699 | -1.02 | 0.9984 | 1.22 | 0.3816 | 1.29 | 0.1902 | 0.0395 |
Nme2 | expressed in non-metastatic cells 2, protein | NM_008705 | 1.01 | 0.9984 | -1.21 | 0.4947 | -1.14 | 0.5423 | 0.0436 |
Pxmp3 | peroxisomal membrane protein 3 | NM_008994 | -1.01 | 0.9984 | 1.11 | 0.3446 | 1.14 | 0.1677 | 0.0242 |
Unc5h3 | Unc5c:unc-5 homolog C (C. elegans), rostral cerebellar malform. | NM_009472 | -1.00 | 0.9984 | -1.08 | 0.6381 | 1.03 | 0.8466 | 0.0475 |