Physical map of the 20 kb fragment cloned from pBMB165 and determination of the minimal replicon. The predicted ORFs are shown by block arrows indicating the direction of transcription. The putative transposable elements are shown by hatched blocks. Replication-related ORFs, an origin of replication (ori165) and iterons region are in black. Other ORFs are shown by white block arrows. The stabilities of the different derivatives in B. thuringiensis are indicated. Inability of the constructs to replicate in B.thuringiensis is indicated by '-'. The fragments obtained by PCR amplification are indicated by the gray lines. The deletion in Rep165 is shown by the open triangle. The frame-shift introduced in orf6 is shown.