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Table 4 Results from hybrid assemblies of BAC 519J4.

From: 454 sequencing put to the test using the complex genome of barley

ABI-Sanger reads total contigsa
50b 47, 48, 44, 47, 46
100b 43, 40, 33, 35, 40
100c 59, 47, 48, 52, 55
200b 40, 31, 35, 36, 40
  1. The 94 sequence contigs provided by 454 Life Sciences Corp (454 sequence contigs). were combined with different numbers of ABI-Sanger reads randomly selected from a set of 1,035 reads. Each assembly was repeated 5 times with different randomly selected sets. Note that the assignment of Phred scale quality values of 40 to the bases in the 454 sequence contigs decreased the number of false collapses considerably while only slightly increasing the overall number of contigs.
  2. aNumber of contigs resulting from 5 repetitions of the assembly with 5 different randomly selected sets of ABI-Sanger sequences.
  3. bBases in 454 sequence contigs were artificially assigned Phred scale quality values of 20.
  4. cBases in 454 sequence contigs were artificially assigned Phred scale quality values of 40.
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