Table 3 Frequency of GCN codons in the second position of HEG and in entire HEG of different organisms. (H0: there is no difference between GCN codon frequencies in the second codon of HEG and in all codons of HEG).
From: Highly expressed proteins have an increased frequency of alanine in the second amino acid position
| Â | Â | percentage of codon | Â | Â | Â | percentage of codon | Â | ||
|---|---|---|---|---|---|---|---|---|---|
organism | codon | 2nd Position of HEG | entire HEG | P-value | organism | codon | 2nd Position of HEG | entire HEG | P-value |
E. coli | GCU | 57.1 | 41.8 | 0.184 | A. fulgidus | GCU | 21.4 | 24.4 | 1.000 |
| Â | GCC | 0.0 | 8.8 | 0.250 | Â | GCC | 21.4 | 22.7 | 1.000 |
| Â | GCA | 42.9 | 25.7 | 0.082 | Â | GCA | 28.6 | 28.0 | 1.000 |
| Â | GCG | 0.0 | 23.7 | 0.007 | Â | GCG | 28.6 | 24.8 | 0.758 |
B. subtilis | GCU | 33.3 | 40.2 | 0.792 | M. jannaschii | GCU | 38.5 | 48.3 | 0.582 |
| Â | GCC | 0.0 | 8.1 | 0.625 | Â | GCC | 0.0 | 4.3 | 1.000 |
| Â | GCA | 53.3 | 31.3 | 0.091 | Â | GCA | 61.5 | 44.7 | 0.268 |
| Â | GCG | 13.3 | 20.4 | 0.749 | Â | GCG | 0.0 | 2.8 | 1.000 |
H. pylori | GCU | 28.6 | 41.3 | 0.419 | P. horikoshii | GCU | 0.0 | 38.0 | 0.049 |
| Â | GCC | 0.0 | 16.6 | 0.144 | Â | GCC | 42.9 | 22.6 | 0.199 |
| Â | GCA | 57.1 | 11.8 | 7.3E-05 | Â | GCA | 28.6 | 31.0 | 1.000 |
| Â | GCG | 14.3 | 30.2 | 0.251 | Â | GCG | 28.6 | 8.4 | 0.113 |
H. influenzae | GCU | 40.0 | 26.1 | 0.199 | S. cerevisiae | GCU | 68.2 | 75.7 | 0.453 |
| Â | GCC | 0.0 | 4.9 | 0.621 | Â | GCC | 22.7 | 23.4 | 1.000 |
| Â | GCA | 60.0 | 50.4 | 0.502 | Â | GCA | 9.1 | 0.6 | 0.010 |
| Â | GCG | 0.0 | 18.7 | 0.037 | Â | GCG | 0.0 | 0.3 | 1.000 |
M. tuberculosis | GCU | 19.2 | 8.0 | 0.054 | S. pombe | GCU | 17.6 | 62.5 | 2.2E-04 |
| Â | GCC | 26.9 | 49.4 | 0.029 | Â | GCC | 5.9 | 31.5 | 0.031 |
| Â | GCA | 26.9 | 7.6 | 0.003 | Â | GCA | 76.5 | 5.3 | 1.4E-13 |
| Â | GCG | 26.9 | 35.0 | 0.535 | Â | GCG | 0.0 | 0.7 | 1.000 |
T. pallidum | GCU | 21.4 | 20.3 | 1.000 | P. falciparum | GCU | 45.5 | 53.6 | 0.763 |
| Â | GCC | 0.0 | 12.2 | 0.397 | Â | GCC | 0.0 | 14.8 | 0.382 |
| Â | GCA | 50.0 | 24.7 | 0.055 | Â | GCA | 45.5 | 31.1 | 0.334 |
| Â | GCG | 28.6 | 42.8 | 0.416 | Â | GCG | 9.1 | 0.4 | 0.061 |
R. prowazekii | GCU | 44.4 | 45.4 | 1.000 | Â | Â | Â | Â | Â |
| Â | GCC | 11.1 | 4.8 | 0.365 | Â | Â | Â | Â | Â |
| Â | GCA | 44.4 | 42.2 | 1.000 | Â | Â | Â | Â | Â |
| Â | GCG | 0.0 | 7.6 | 1.000 | Â | Â | Â | Â | Â |
M. genitalium | GCU | 33.3 | 48.9 | 0.507 | Â | Â | Â | Â | Â |
| Â | GCC | 0.0 | 7.2 | 1.000 | Â | Â | Â | Â | Â |
| Â | GCA | 66.7 | 39.8 | 0.168 | Â | Â | Â | Â | Â |
| Â | GCG | 0.0 | 4.1 | 1.000 | Â | Â | Â | Â | Â |
B. burgdorferi | GCU | 22.2 | 50.3 | 0.177 | Â | Â | Â | Â | Â |
| Â | GCC | 0.0 | 8.0 | 1.000 | Â | Â | Â | Â | Â |
| Â | GCA | 77.8 | 36.5 | 0.015 | Â | Â | Â | Â | Â |
| Â | GCG | 0.0 | 5.2 | 1.000 | Â | Â | Â | Â | Â |