Figure 4

Experimental results for Rpl10 (a-b) and Stk23 (c-d). a. In situ hybridisation of Rpl10 on adult and embryonic tissues. Upper panels: antisense probe, lower panels: sense probe. 1 hypothalamus with signal in the nucleus of a sagittal brain section, 2 adult testis with signal in leydig cells, 3 epididymis, 4 dpc 16.5 intestine, 5 dpc 16.5 kidney, 6 dpc 16.5 skeletal system/clavicle. b. Northern hybridisation with a Rpl10-specific probe on a self-made MTN blot, 15 μg of murine total RNA per lane. c. Northern hybridisation with a Stk23-specific probe on Clontech adult and embryonic MTN blots. d. RNA in situ hybridisation of Stk23 in different embryonic stages. 1 dpc 10.5 heart, 2 dpc 12.5 intercostal muscles, 3 dpc 14.5 tongue and heart, 4 dpc 14.5 intercostal muscles.