Table 1 Summary of screening results of the giant panda BAC library by PCR with primers for microsatellites
Microsatellite Sequence Name | Primer (5'-3') | Ta (°C) | Product length (bp) | Positive superpool number |
|---|---|---|---|---|
A204 | F- CCTGCCCTATGGATTTCAGA R-GGTCCATATCATGGTCTAAATGA | 63 | 321 | 10 |
A066 | F- GATTATCCGTTGGCTTTGGA R- CGTGGTTGTCTCCACACTTG | 60 | 337 | 3 |
B043 | F- GACCCTGTGGCTCTGTGACT R- GCGAGGGAAGGAAGAGAAAC | 60 | 310 | 6 |
GPMC-1 | F- AGAGAGAGAGCGAGCACGAG R- AGCCACGAAACAGAGAGGAA | 63 | 207 | 1 |
GPMC-21 | F- GAATGGTATGCCTGGGTGAC R- AACACAAGCGGGGGAACT | 63 | 150 | 6 |
GPMC-40 | F- TCCCTCAGCACCTTAACACC R- TACTGGCCCAGGACACTAGC | 58 | 214 | 4 |
GPMC-52 | F- CCGTCTCCAAGGTAACCTGA R- AGAGATTGCTAGGCGGACAA | 60 | 190 | 3 |
GPMC-104 | F- TCTCCATCAGGTTACCACTGC R- CTGCGTGCCTCAGTCAGTTA | 60 | 261 | 4 |