Table 2 Summary of screening results of the giant panda BAC library by PCR with primers for functional genes
Gene | Genebank accession No. | Primers (5'-3') | Ta (°C) | PCR product size (bp) | Positive clone number |
|---|---|---|---|---|---|
ACTIVIN | AF350256 | F- GGACATTGGCTGGAATGACT R- GACATGGGTCTCAGCTTGGT | 54 | 207 | 4 |
AGXT | AJ539311 | F- CTGTTTTTCATTGACTGGACTCTG R- TCTCATCCATTATCTGGGGG | 58 | 539 | 4 |
GHR | AF367203 | F- CCTCTGGGGGAAAAAAAGTT R- TCATCATCCTTTGCTCCAAG | 56 | 412 | 8 |
FSH | AF448454 | F- TGTTGCTGGAGAGCAATCTG R- TACGCCAGCTCTTTGAAGGT | 54 | 197 | 7 |
FSHR | AF415208 | F- GCCAAAGGATTTGACACGAT R- TGGTGACCAGGATCATCAGA | 56 | 202 | 6 |
GpH | AF448453 | F- GCCCAGAATGCAAGCTAAAG R- ACTGTGGCCTTGGTAAATGC | 54 | 190 | 5 |
SOX14 | AF016377 | F- AACCCCAAGATGCACAACTC R- ACGTAGCCTCTTGGCCTCAC | 54 | 108 | 6 |
IRBP | AY303836 | F- CCAGGAAGTGGTGAGCAAGC R- CGGTGAGGAAGAAGTCGGAC | 66 | 419 | 4 |
TTR | AF039738 | F- TCTGCAGAGCTCACTGAACC R- TTCGATTAGCTGAGGGGAAA | 54 | 205 | 4 |
BDNF | U56638 | F- TCTGGAGAGCGTGAATGGG R- CGATACAGGGACTTTTTCAAGG | 62 | 394 | 3 |
GDNF | AF516767 | F- TGAAAAGGTCACCCGAGAAG R- AGCCGCTGCAGTACCTAAAA | 54 | 219 | 5 |
NT-4 | AY189135 | F- AGCGAGACAGCACCAGCAA R- TCAGACACCCAGTGCCTCC | 64 | 263 | 5 |
LH | AF448455 | F- GCTGTGGCTGCTGCTGAAC R- CCACAGGGAAGGAGACCATG | 58 | 313 | 2 |
ZFX1 | AF016379 | F- TCGGTTCACACGAAGGACTAC R- TGATACACCTTCCTGCCACTG | 59 | 284 | 4 |
ZFX2 | AF016380 | F- GGTGCAGCAACATGCTCTTA R- CCACGTGTTTCTTGAGTTCG | 54 | 188 | 4 |