Figure 2
Overview of mouse and malaria gene expression in all organs. A. Highly expressed mouse genes. 2268 mouse genes were expressed in the top 25th percentile. Left, normalized intensity values of genes (in rows) are ordered using Pearson correlation and hierarchical clustering. Samples (columns) are grouped chronologically (i.e. Day 0, 3, and 6) by organ. Right, GO-gram indicates statistically significant enrichments of select GO-BP functional annotations in gene clusters. Red bars indicate that a particular gene is annotated with the given GO-BP category and that the GO-BP category is significantly over-represented within the cluster in which the gene was placed. Blue bars indicate that a particular gene is annotated with the given GO category and that the GO category is not significantly enriched within the given cluster. Not all GO categories that displayed significant enrichment are shown. For brevity, GO-BP category names are abbreviated and final digits of GO identifiers are shown in parentheses. The vertical multi-colored bar denotes gene clusters (pink indicates genes highly expressed in the lung, blue in brain, yellow in liver and grey in spleen). GO-BP categories overrepresented in each of the known organs correlate with known function, e.g. GO-BP categories significantly enriched in the spleen cluster include "immune response" and "response to biotic stimulus". B. Highly expressed malaria genes. 2159 malaria genes were expressed in the top 25th percentile. Left, normalized intensity values of genes were ordered using Pearson correlation and hierarchical clustering. Samples are grouped chronologically by organ. Middle and Right, GO- and Protein-gram indicate statistically significant enrichments of GO-BP or Pfam annotations in highlighted gene clusters. Color codes are as in A. Interestingly, the majority of highly expressed PbA genes were found in the lung and functional annotations for these transcripts included categories related to metabolism or host-parasite interaction.