Figure 4

A network involving genes differentially expressed at Day0 may be associated with protective early immune response to infection. A. Genes differentially expressed at baseline in liver were found to be significantly overrepresented in this network by the Ingenuity Pathway Analysis software (P < 0.05). This network involves the transcription factor stat1 and many other immunologically important transcripts encoding cell adhesion molecules (icam1, vcam1), receptors (tlr8,ifnar2) acute phase proteins (crp, apcs) and others (cxcl9, gbp1, ikbke). Increasing colour intensities indicate relative up-regulation of gene expression in resistant (red nodes) compared to susceptible mice (green nodes). B. Clustergram showing log2-scale ratios of BALB/c to B6 intensity values of the genes found in the network (A). Genes (in rows) are grouped using cosine-angle correlation and hierarchical clustering, and samples (columns) are chronologically ordered by organ. Many of these genes which were upregulated in BALB/c liver at baseline become up-regulated in susceptible (B6) mice over the course of infection.