Figure 5

Malaria genes differentially expressed across the infection time course in resistant and susceptible mice. 439 PbA genes were selected as differentially expressed in resistant and susceptible hosts during infection (linear model, P < 0.05). Left, log2-ratios of BALB/c to B6 intensity measurements of PbA genes (rows) are clustered using cosine-angle correlation. Samples (in columns) are chronologically ordered by organ. Middle, intensities (normalized and median-subtracted) are shown for each gene in each mouse strain. Eight major clusters are highlighted on the dendrogram and using a vertical, multi-colored bar. Right, GO- and Protein-grams indicating statistically significant enrichments of GO-BP or Pfam annotations in the highlighted clusters. GO-BP and Pfam names are abbreviated and numbers in parenthesis correspond to final digits of GO identifiers. Red bars indicate that a particular gene is annotated with the given category and that the category is significantly over-represented within the cluster in which the gene was placed. Blue bars indicate that a particular gene is annotated with the given category and that the category is not significantly enriched within the given cluster. Not all GO and Pfam categories that displayed significant enrichment are shown. Lower panel, detailed view of representative clusters including highlighted genes of interest. The clusters identify gene groups that show remarkable differences in expression between hosts and also between tissues, giving evidence that PbA transcription is influenced both by the host response and tissue microenvironment.