Exploring glycopeptide-resistance in Staphylococcus aureus: a combined proteomics and transcriptomics approach for the identification of resistance-related markers

Table 6 List and characteristics of primers and probes used for validation of identified markers.

Gene number^A	Sequence (5'→3')	Length	5'-Dye	Concentration (nM)
*SA0591*
F_SA0591-195F	TGGCCGTGCAAAAGATTTAGT	21		200
R_SA0591-275R	TGTGTAATTGCGAATCCTTGTTG	23		200
P_SA0591-219T	TGTAACAAGTCCACGAGAGCGACAACAGA	29	FAM	100
*SA2113*
F_SA2113-40F	GCCGGTTTAAATGACCGTACTACT	24		200
R_SA2113-117R	TTGCAACGCATCACAAAATTG	21		200
P_SA2113-75T	CACTTTTGCATAACTTACTT^B	20	VIC	100
*SA1195*
F_SA1195-799F	CCTGATTCAGGGATTTTCCAAA	22		200
R_SA1195-877R	TTGGCACAGTCAATGACTTAACATC	25		200
P_SA1195-822T	TCACCTCGGATGCCAAAACTCAAACCT	27	FAM	100
*SA1691*
F_SA1691-319F	CATCATGGATTCGATTTGAAAGG	23		200
R_SA1691-389R	CCTTGCACATCTCTGTCGCTAA	22		200
P_SA1691-344T	CAACTAGAGCTTTATTTTCA^B	20	FAM	100
*SA0536.1*
F_SA0536.1-31F	GGCGCACCAGTTTATGAAATTAT	23		200
R_SA0536.1-111R	TTCAGTATCACTAAATGAATCGTCACAT	28		200
P_SA0536.1-60T	AACGTTTCAGCATGTTT^B	17	VIC	100
*SA0532*
F_SA0532-15F	GATTGCGATAACTTCTTTTCTCTAT	25		200
R_SA0532-115R	AATCAAACTTAAGCCCTAGATAAA	24		200
P_SA0532-51T	CAGTATCAAATTTATCTAGGGCTTA^B	25	VIC	100
*rRNA16S*
F_rRNA16s-551F	GGCAAGCGTTATCCGGAATT	20		200
R_rRNA16S-651R	GTTTCCAATGACCCTCCACG	20		200
P_rRNA16s-573T	CCTACGCGCGCTTTACGCCCA	21	FAM	100

^ABased on the annotation of N315 genome.
^B Minor groove binder (MGB) probes with non-fluorescent quencher bound to the 3'-end (Applied Biosystems).