Figure 6
From: Comparative analysis of dinoflagellate chloroplast genomes reveals rRNA and tRNA genes
RT-PCR analysis of LSU and SSU rRNA sequences. a) Lane 1, Hyperladder IV markers (Bioline); Lane 2, Hyperladder I markers (Bioline); Lane 3, RT-PCR with primers LSU1F and LSU2R and A. operculatum RNA template; Lane 4, as Lane 3 but no RT enzyme; Lane 5, PCR with primers LSU1F and LSU2R and A. operculatum DNA template; Lane 6, as Lane 5 but no DNA template added; Lane 7, RT-PCR with primers LSU3F and LSU4R and A. operculatum RNA template; Lane 8, as Lane 7 but no RT enzyme; Lane 9, PCR with primers LSU3F and LSU4R and A. operculatum DNA template; Lane 10, as Lane 9 but no DNA template added. b) Lane 1, Hyperladder I markers (Bioline); Lane 2, RT-PCR with primers SSU1F and SSU2R and A. operculatum RNA template; Lane 3, as Lane 2 but no RT enzyme; Lane 4, PCR with primers SSU1F and SSU2R and A. operculatum DNA template; Lane 5, as Lane 4 but no DNA template added.