Table 2 PCR markers analyzed and their replication timing.
From: Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts
Primer set name | Sequence | Product range | Replication time in hrs1(fibroblasts) | Woodfine replication timing ratio2 |
|---|---|---|---|---|
chr6.1385A | F ctcagctttccctgttaatg R ggaagatgctaaatgactgc | 30904535–30904712 | 1.0 | 1.73 |
chr6.1385B | F ggaattaaggcgtgtatctg R aacttcccatagggattagc | 31023340–31023694 | 1.5 | 1.63 |
chr6.1386A | F agtaaatccgggtctctagg R ccagatagaggcactgagag | 32021221–32021449 | 1.0 | 1.74 |
chr6.1386B | F aaatgtccttcaccatcaag R attatcagagcagcaaaagg | 32151938–32152179 | 0.9 | 1.74 |
chr6.1395A | F atgactcatgtagggcagac R aaggaggaacaagaggaaac | 40489161–40489555 | 5.6 | 1.36 |
chr6.1395B | F gtccaagctaagtccatgag R gatgacaaacatgaatgcag | 40560441–40560763 | 5.5 | 1.25 |
chr6.1395C | F taggtcagttgacccatctc R cgtgttcagtgtgtatttgc | 40601430–40601688 | 5.4 | 1.58 |
chr6.1401A | F caggcccatacttcttgtag R gtgagagcttctccttgatg | 43125237–43125402 | 1.6 | 1.73 |
chr6.1401B | F tggttcttctctccagtttg R taggtgaggacacaagatcc | 43237884–43238178 | 2.1 | 1.73 |
chr6.1417A | F cagtagtgtgtgcacctgtc R tccctgtaacttcaaccatc | 89898270–89898634 | 4.6 | 1.51 |
chr6.1417B | F taaccccactctgtgttagg R cctatggagtcagagattgc | 89963481–89963615 | 4.8 | 1.51 |
chr22.1096A | F agggtcacatctacagttgg R ccatttgctgtcctttctac | 23123485–23123611 | 1.1 | 1.85 |
chr22.1096B | F aatcctcctgagaattaggc R caggagtaatgcccacttag | 23239758–23239902 | 0.5 | 1.72 |
chr22.1102A | F tcaggaggagtttcacattc R cacagaagactcagggagag | 26718830–26718968 | 1.0, 5.0 | 1.58 |
chr22.1102B | F gctaggacctgaactcacac R tttaaagggctgctctattg | 26841004–26841279 | 5.6 | 1.55 |
chr22.1110A | F gccctgagatactgactctg R aaatgcagaatgaagtggag | 31334872–31335235 | 1.0 | 1.55 |
chr22.1110B | F gacattgctcttgcctctac R cttctcaagggtgtaaatgg | 31441419–31441684 | 1.0 | 1.54 |
chr22.1112A | F caggcatctgaaattaaacc R agattagaggctggttttcc | 32486488–32486736 | 6.0 | 1.68 |
chr22.1112B | F actgagattcaaaccagagc R agggaaacttggtaatagcc | 32533028–32533232 | 6.1 | 1.64 |
chr22.1118A3 | F ttcagggtctggttggtagg R agcaggagactggcacagat | 38071800–38072021 | 0.8 | 1.99 |
chr22.1118B | F gtggggtgcactgttactat R atcagggacacgtaaacaac | 38181333–38181482 | 0.8 | 1.95 |