Figure 3

Four clusters illustrating transcriptional responses to MMS and/or γ-ray. S. cerevisiae cultures were exposed to one of three doses of MMS (0,001%, 0.01%, or 0.1% (v/v)) or one of three doses of γ-ray (1 Gy, 10 Gy, or 100 Gy) and their global transcriptional response was compared to that of control cells not exposed to any DNA damaging agents. All genes clustered had at least a 3-fold change in expression level to one or more experimental conditions. These four clusters had particularly interesting responses to MMS and γ-ray: (A) 3 genes repressed by all doses of MMS and γ-ray, (B) 2 genes repressed by all doses of MMS and induced by all doses of γ-ray, (C) 2 genes induced by all doses of MMS and repressed by γ-ray and (D) 54 genes induced by all doses of MMS and γ-ray.