Figure 5

Conservation of amino acid binding signature in the OlfC family. Sequence logos were constructed for predicted binding pocket residues in the zebrafish OlfC (drOlfC) and mouse V2R (mmV2R) receptor families, based on an alignment of full-length, intact zebrafish OlfC and mouse V2R amino acid sequences. The over-representation of amino acids at positions equivalent to those predicted to interact with amino acid ligands in zebrafish OlfCa1 [28] is shown graphically. Y axis, information content; X axis, residue position with respect to OlfCa1 (note that positions are non-contiguous). Conserved proximal pocket signature motif sites that are thought to make contact with the ligand are highlighted in bold black, whereas predicted distal binding pocket sites are shown in grey. Additional signature motif residues thought to play a structural role but not contact ligand directly are shown in bold blue (bottom of figure; see the text for details).