Table 2 Matching analysis of MPSS and RL-SAGE tags to M. grisea EST and genomic sequences.
MgRefSeq1 | Library | MPSS | RL-SAGE | ||||
|---|---|---|---|---|---|---|---|
| Â | Â | Appressoria | Mycelia | Mycelia | |||
| Â | Â | Significant tags (%) | Non Significant tags (%) | Significant tags (%) | Non Significant tags (%) | Significant tags (%) | Non Significant tags (%) |
Genomic DNA | Both sense + antisense | 10,957 (84.8) | 2,335 (66.7) | 10,649 (85.0) | 5,912 (76.1) | 10,772 (65.0) | 8,950 (25.3) |
ESTs | Sense | 5,597 (43.3) | 753 (21.5) | 5,557 (44.3%) | 1,980 (25.5) | 3,684 (22.2) | 2,218 (6.3) |
| Â | Antisense | 1,185 (9.2) | 415 (11.8) | 2,374 (18.9) | 1,912 (24.6) | 1,572 (9.5%) | 1,419 (4.0) |
| Â | Both sense + antisense | 6,620(51.2) | 1,149 (32.8) | 7,750 (61.8) | 3,842(49.5) | 5,067 (30.6) | 3,567 (10.0) |
Coding region (CDS) | Sense | 4,574 (35.4) | 690 (19.7) | 4,761 (38.0) | 1,935 (24.9) | 2,058 (12.4) | 1,751 (5.0) |
| Â | Antisense | 653 (5.1) | 329 (9.4) | 1,825 (14.6) | 1,837 (23.6) | 605 (3.6) | 890 (2.5) |
| Â | Both sense + antisense | 5,205 (40.3) | 1,016 (29.0) | 6,560 (52.4) | 3,759 (48.4) | 2,648 (16.0) | 2,638 (7.5) |
500 bp upstream sequences2 | Sense | 1,730 (13.4) | 367 (10.5) | 1,352 (10.8) | 715 (9.2) | 2,548 (7.2) | 1,399 (4.0) |
| Â | Antisense | 699 (5.4) | 284 (8.1) | 906 (7.2) | 655 (8.4) | 2,495 (7.0) | 1,204 (3.4) |
| Â | Both sense + antisense | 905 (7.0) | 301 (8.6) | 877 (7.0) | 630 (8.1) | 964 (5.8) | 962 (2.7) |
500 bp downstream sequences3 | Sense | 4,075 (31.5) | 618 (17.7) | 2,986 (23.8) | 1,097 (14.1) | 3,117 (6.0) | 4,842 (29.2) |
| Â | Antisense | 3,687 (28.5) | 581 (16.6) | 3,044 (24.3) | 1,037 (13.4) | 2,251 (4.3) | 3,997 (24.1) |
| Â | Both sense + antisense | 4,189(32.4) | 600 (17.1) | 3,065 (24.5) | 1,005 (12.9) | 5,131 (30.9) | 2,799 (7.9) |
Number of genes transcribed4 | - | 7,686 | 2,115 | 6,735 | 4,234 | 6,029 | 5,163 |
Novel transcript tags-TIGR ESTs5 | - | 4,478 (34.64) | 1,220 (34.85) | 3,297 (26.3) | 2,281 (29.4) | 5,887 (35.5) | 5,499 (15.55) |
Novel transcript tags-COGEME ESTs6 | - | 7,573 (58.6) | 1,876 (53.6) | 6,737 (53.8) | 4,607 (59.3) | 7,366 (44.4) | 7,167 (20.3) |
Putative intergenic tags7 | - | 1,618 (12.5) | 571 (16.3) | 1,211 (9.7) | 977 (12.6) | 2,716 (16.4) | 3,004 (8.5) |
Putative intergenic tags match ESTs8 | - | 394 (3.0) | 70 (2.0) | 395 (3.1) | 189 (2.4) | 596 (3.6) | 437 (1.2) |