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Table 2 Matching analysis of MPSS and RL-SAGE tags to M. grisea EST and genomic sequences.

From: Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods

MgRefSeq1 Library MPSS RL-SAGE
   Appressoria Mycelia Mycelia
   Significant tags (%) Non Significant tags (%) Significant tags (%) Non Significant tags (%) Significant tags (%) Non Significant tags (%)
Genomic DNA Both sense + antisense 10,957 (84.8) 2,335 (66.7) 10,649 (85.0) 5,912 (76.1) 10,772 (65.0) 8,950 (25.3)
ESTs Sense 5,597 (43.3) 753 (21.5) 5,557 (44.3%) 1,980 (25.5) 3,684 (22.2) 2,218 (6.3)
  Antisense 1,185 (9.2) 415 (11.8) 2,374 (18.9) 1,912 (24.6) 1,572 (9.5%) 1,419 (4.0)
  Both sense + antisense 6,620(51.2) 1,149 (32.8) 7,750 (61.8) 3,842(49.5) 5,067 (30.6) 3,567 (10.0)
Coding region (CDS) Sense 4,574 (35.4) 690 (19.7) 4,761 (38.0) 1,935 (24.9) 2,058 (12.4) 1,751 (5.0)
  Antisense 653 (5.1) 329 (9.4) 1,825 (14.6) 1,837 (23.6) 605 (3.6) 890 (2.5)
  Both sense + antisense 5,205 (40.3) 1,016 (29.0) 6,560 (52.4) 3,759 (48.4) 2,648 (16.0) 2,638 (7.5)
500 bp upstream sequences2 Sense 1,730 (13.4) 367 (10.5) 1,352 (10.8) 715 (9.2) 2,548 (7.2) 1,399 (4.0)
  Antisense 699 (5.4) 284 (8.1) 906 (7.2) 655 (8.4) 2,495 (7.0) 1,204 (3.4)
  Both sense + antisense 905 (7.0) 301 (8.6) 877 (7.0) 630 (8.1) 964 (5.8) 962 (2.7)
500 bp downstream sequences3 Sense 4,075 (31.5) 618 (17.7) 2,986 (23.8) 1,097 (14.1) 3,117 (6.0) 4,842 (29.2)
  Antisense 3,687 (28.5) 581 (16.6) 3,044 (24.3) 1,037 (13.4) 2,251 (4.3) 3,997 (24.1)
  Both sense + antisense 4,189(32.4) 600 (17.1) 3,065 (24.5) 1,005 (12.9) 5,131 (30.9) 2,799 (7.9)
Number of genes transcribed4 - 7,686 2,115 6,735 4,234 6,029 5,163
Novel transcript tags-TIGR ESTs5 - 4,478 (34.64) 1,220 (34.85) 3,297 (26.3) 2,281 (29.4) 5,887 (35.5) 5,499 (15.55)
Novel transcript tags-COGEME ESTs6 - 7,573 (58.6) 1,876 (53.6) 6,737 (53.8) 4,607 (59.3) 7,366 (44.4) 7,167 (20.3)
Putative intergenic tags7 - 1,618 (12.5) 571 (16.3) 1,211 (9.7) 977 (12.6) 2,716 (16.4) 3,004 (8.5)
Putative intergenic tags match ESTs8 - 394 (3.0) 70 (2.0) 395 (3.1) 189 (2.4) 596 (3.6) 437 (1.2)
  1. 1 Magnaporthe grisea reference sequences.
  2. 2 Probably 5'UTR region.
  3. 3 Probably 3'UTR region.
  4. 4Number of genes (including single and multiple hits) expressed using CDS, 500 bp upstream and downstream sequences.
  5. 5Tags matched to the genome sequence but not to TIGR-M. grisea ESTs.
  6. 6Tags match to the genome sequence but not to COGEME M. grisea ESTs.
  7. 7Tags matched to the genomic sequence but not to the CDS, 500 bp upstream or downstream sequences.
  8. 8Putative intergenic tags that match to M. grisea ESTs deposited either in TIGR or COGEME