Figure 2

Transcript analysis by Northern-blot hybridization. Total RNA from epididymis (E) and spleen (S) were hybridized with cDNA probes of the genes. Agarose gels were stained with ethidium bromide to visualize 28S and 18S RNAs as a control to ensure loading of the same amount of RNA in each lane. There were significant differences in transcript size between the Northern blots and the UniGene predictions for eight genes, and these genes were subjected to RACE. This resulted in an additional new sequence for a gene (Mm.335028). Transcript sizes from known sequences (UniGene database and RACE), transcripts with significant differences in size between the Northern blots and cDNA sequences, and transcripts with isoforms are indicated below the blots. In the RACE analysis, genes with results in which transcript sequences were not extended (N.E.) or no result (N.R.), i.e. no amplification of transcript sequence in the analysis, are indicated. In the case of results from both 5'- and 3'-RACE in a given gene, the results are shown as 5'/3'.