Figure 7
Secretion of proteins encoded by the novel genes. COS-7 cells were transfected with pcDNA3.1-UniGene-myc/His. After 48 hours, UniGene-myc/His were immunoprecipitated with anti-myc mAb from the culture medium and the cell lysates, and then subjected to Western-blot analysis using α-myc. Proteins were divided into two groups based on their secretion profile: secretory (A) and intracellular (B) proteins. Vector alone (Mock) and cysteine-rich secretory protein 1 (CRISP1) were used as a negative control and secretion marker, respectively. The lower bands in the immunoblot of Mm.99576 represent immunoglobulin G (IgG) heavy chain. The arrow indicates the molecular weight of the each protein.