Figure 3

Northern analysis of genes related to amino acid biosynthesis and glutathione metabolism in T. reesei. The expression level of the genes was analysed in bioreactor cultures treated with DTT for 0, 30 and 60 min and in untreated reference cultures, in chemostat cultures of a tPA producing strain and its parental strain, and in a strain overexpressing ire1 and in its parental strain. The signals of the specific mRNAs were quantified and normalised using the signals of gpd1. The ratio of the signal in the analysed sample vs. the signal in a corresponding reference culture are shown. T. reesei protein model identifier (genome version 1.2), likely S. cerevisiae homologue and amino acid for whose biosynthesis or use the gene is involved is indicated after the gene name. DTT 0 min, DTT treatment 0 min; DTT 30 min, DTT treatment 30 min; DTT 60 min, DTT treatment for 60 min; tPA, tPA producing chemostat culture; ire1, ire1 overexpressing strain, AA transport, amino acid transport.