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Table 2 The most abundant ORFs in the secretion stress related ESTs of T. reesei. 25 ORFs represented by the highest number of ESTs in the cDNA subtraction libraries or cDNA-AFLP fragments derived from the secretion stress conditions are listed. Gene names from T. reesei (small case letters) or S. cerevisiae (capital letters) are shown. Description and T. reesei protein model identifiers (ID) are from T. reesei genome version 1.2 website. The number of ESTs corresponding to the genes in the libraries are shown: "AFLP", fragments from cDNA-AFLP analysis induced in both tPA expression and DTT treatment; "DTThyb1", subtraction library from DTT treated culture after one subtraction round; "DTTHyb5", subtraction library from DTT treated culture after five subtraction rounds; "tPAhyb1", subtraction library from the tPA producing culture after one subtraction round; "tPAHyb5", subtraction library from tPA producing culture after five subtraction rounds.

From: Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae

Gene

T. reesei protein Model ID

Description

AFLP

DTThyb1

DTThyb5

tPAhyb1

tPAhyb5

Total

TEF2

tre41793

translational elongation

 

25

12

42

18

97

bip1

tre42955

HSP70 family ER chaperone

1

55

1

1

 

58

 

tre42349

posttranslational modification, protein turnover

 

40

15

1

2

58

UBI4

tre45056

posttranslational modification, protein turnover

 

46

2

1

2

51

cbhII

tre44954

cellobiohydrolase II

   

40

10

50

FRE4

tre39576

Ferric reductase-like transmembrane component

 

26

 

7

 

33

URA8

tre13936

phospholipid biosynthesis, pyrimidine base biosynthesis

 

1

  

32

33

cbhI

tre22421

cellobiohydrolase I

   

24

8

32

cpc1

tre37844

basic-leucine zipper (bZIP) transcription factor

 

21

 

10

 

31

 

tre44646

unknown

 

13

12

1

1

27

 

tre43768

ankyrin

 

13

11

 

2

26

 

tre46310

putative exoglucanase

 

1

 

8

15

24

ACT1

tre41819

actin

 

8

2

8

5

23

ITR2

tre43977

intergal membrane myo-inositol transporter

1

  

16

2

19

 

tre45769

aromatic compound metabolism

 

19

   

19

 

tre43393

ADP-ribosylglycohydrolase

 

9

8

 

1

18

prp2

tre42899

putative PDI-related protein

 

2

 

3

13

18

MBF1

tre42770

transcription factor MBF1

 

13

3

 

1

17

HTA2

tre35558

histone H2A

 

9

4

1

2

16

YLL023C

tre43299

putative ER membrane protein

 

10

5

  

15

pdi1

tre45146

ER-resident foldase

 

12

 

2

 

14

cip1

tre41957

esterase homologue

   

6

8

14

 

tre45203

Transcriptional coactivator p100

 

4

7

2

1

14

hex1

tre45186

woronin body

 

1

 

10

2

13

VPS1

tre44901

vacuolar sorting protein

 

12

  

1

13

Amount of ESTs sequenced from the individual libraries

94

961

240

510

242

2047