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Table 2 The most abundant ORFs in the secretion stress related ESTs of T. reesei. 25 ORFs represented by the highest number of ESTs in the cDNA subtraction libraries or cDNA-AFLP fragments derived from the secretion stress conditions are listed. Gene names from T. reesei (small case letters) or S. cerevisiae (capital letters) are shown. Description and T. reesei protein model identifiers (ID) are from T. reesei genome version 1.2 website. The number of ESTs corresponding to the genes in the libraries are shown: "AFLP", fragments from cDNA-AFLP analysis induced in both tPA expression and DTT treatment; "DTThyb1", subtraction library from DTT treated culture after one subtraction round; "DTTHyb5", subtraction library from DTT treated culture after five subtraction rounds; "tPAhyb1", subtraction library from the tPA producing culture after one subtraction round; "tPAHyb5", subtraction library from tPA producing culture after five subtraction rounds.

From: Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae

Gene T. reesei protein Model ID Description AFLP DTThyb1 DTThyb5 tPAhyb1 tPAhyb5 Total
TEF2 tre41793 translational elongation   25 12 42 18 97
bip1 tre42955 HSP70 family ER chaperone 1 55 1 1   58
  tre42349 posttranslational modification, protein turnover   40 15 1 2 58
UBI4 tre45056 posttranslational modification, protein turnover   46 2 1 2 51
cbhII tre44954 cellobiohydrolase II     40 10 50
FRE4 tre39576 Ferric reductase-like transmembrane component   26   7   33
URA8 tre13936 phospholipid biosynthesis, pyrimidine base biosynthesis   1    32 33
cbhI tre22421 cellobiohydrolase I     24 8 32
cpc1 tre37844 basic-leucine zipper (bZIP) transcription factor   21   10   31
  tre44646 unknown   13 12 1 1 27
  tre43768 ankyrin   13 11   2 26
  tre46310 putative exoglucanase   1   8 15 24
ACT1 tre41819 actin   8 2 8 5 23
ITR2 tre43977 intergal membrane myo-inositol transporter 1    16 2 19
  tre45769 aromatic compound metabolism   19     19
  tre43393 ADP-ribosylglycohydrolase   9 8   1 18
prp2 tre42899 putative PDI-related protein   2   3 13 18
MBF1 tre42770 transcription factor MBF1   13 3   1 17
HTA2 tre35558 histone H2A   9 4 1 2 16
YLL023C tre43299 putative ER membrane protein   10 5    15
pdi1 tre45146 ER-resident foldase   12   2   14
cip1 tre41957 esterase homologue     6 8 14
  tre45203 Transcriptional coactivator p100   4 7 2 1 14
hex1 tre45186 woronin body   1   10 2 13
VPS1 tre44901 vacuolar sorting protein   12    1 13
Amount of ESTs sequenced from the individual libraries 94 961 240 510 242 2047