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Table 1 Basic features of the three polymorphic markers on Coja region

From: The major histocompatibility complex (Mhc) class IIB region has greater genomic structural flexibility and diversity in the quail than the chicken

 

Coja haplotype 1 (Acc. Num. AB078884)

    

marker name

Nuc. position**

Gene position

length (bp)

range (bp)

polymorphisms

primer name

primer sequence (5' to 3')

PM1

89043 – 89887

Tapasin (ex.4 – int.4)

845

718–1095

(ATGAT)n

(TTCCTATGGGGGCTGTAGGGTGGATGGGACTGGGTGGTA)n

TAPBPL-F

CAGGTCCTGCTGGCCTATGA

      

TAPBPL-R

TGGTGTGATGCCAACCCAT

PM2

92768 – 93787

Coja-DBB1 (Promoter – ex.3)

1002

989–1002

126 SNPs, 13 indels

CojaDBBpromoterF6

CCCTGGGGACACCATTTGTCAT

      

C2BNO5

GCGCCAGGAAGACGAGCCCCAGCAC

PM3*

118204 – 118536

DMB2 (int.1 – int.2)

333

333

G/A, G/C

DMB2-F

GGAGTGCATCCCCATTGCT

      

DMB2-R

GCTCACTCTTGCGCAGTGC

  1. * PM3 contains two SNP sites.
  2. ** positions on Coja haplotype 1 genomic sequence (accession number; AB078884).
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BMC Genomics

ISSN: 1471-2164

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