Table 4 Results of validation by qRT-PCR alongside detection of differential expression by microarray demonstrating qualitative agreement.
qPCR Validation | ||||||||
|---|---|---|---|---|---|---|---|---|
Gene Name | 2ΔΔCT | 2ΔΔCT standard deviation | qPCR p-value | qPCR | Direction of Imprinted Expression | Porcine Spotted Long Oligo | Human Short Oligo | Porcine Short Oligo |
PEG10 | 785544.42 | 9.3 | <0.0001 | Yes | Paternal | Yes | Yes | Yes |
PLAGL1 | 758.32 | 1.4 | <0.0001 | Yes | Paternal | No | Yes | Yes |
SGCE | 450.90 | 2.1 | <0.0001 | Yes | Paternal | No | Yes | Yes |
IGF2 | 24.53 | 1.9 | 0.0010 | Yes | Paternal | No | Yes | Yes |
DLX5 | 7.53 | 1.5 | 0.0065 | Yes | Maternal | No | No | No |
DCN | 5.04 | 1.6 | 0.0168 | Yes | Maternal | No | No | No |
GRB10 | 1.38 | 2.2 | 0.1913 | No | Maternal | No | No | No |
CD81 | 0.98 | 1.6 | 0.2026 | No | Maternal | No | No | No |
COMMD1 | 0.86 | 2.8 | 0.3265 | No | Maternal | No | No | No |
CDKN1C | 0.79 | 6.7 | 0.4283 | No | Maternal | No | No | No |
IGF2R | 0.74 | 1.3 | 0.2119 | No | Maternal | No | No | No |