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Table 4 Results of validation by qRT-PCR alongside detection of differential expression by microarray demonstrating qualitative agreement.

From: Detection of transcriptional difference of porcine imprinted genes using different microarray platforms

qPCR Validation
Gene Name 2ΔΔCT 2ΔΔCT standard deviation qPCR p-value qPCR Direction of Imprinted Expression Porcine Spotted Long Oligo Human Short Oligo Porcine Short Oligo
PEG10 785544.42 9.3 <0.0001 Yes Paternal Yes Yes Yes
PLAGL1 758.32 1.4 <0.0001 Yes Paternal No Yes Yes
SGCE 450.90 2.1 <0.0001 Yes Paternal No Yes Yes
IGF2 24.53 1.9 0.0010 Yes Paternal No Yes Yes
DLX5 7.53 1.5 0.0065 Yes Maternal No No No
DCN 5.04 1.6 0.0168 Yes Maternal No No No
GRB10 1.38 2.2 0.1913 No Maternal No No No
CD81 0.98 1.6 0.2026 No Maternal No No No
COMMD1 0.86 2.8 0.3265 No Maternal No No No
CDKN1C 0.79 6.7 0.4283 No Maternal No No No
IGF2R 0.74 1.3 0.2119 No Maternal No No No