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Table 2 Linear regression analysis of the 2-fold dilution data

From: Evaluation of low density array technology for quantitative parallel measurement of multiple genes in human tissue

Gene Mean Ct Slope R2 Gene Mean Ct Slope R2
18S-4342379-18S 14.71 -2.72 0.96 CD62E (SELE)-Hs00174057_m1 31.55 -6.53 0.93
C3-Hs00163811_m1 19.83 -3.92 0.97 IL-8-Hs00174103_m1 31.55 -5.83 0.87
FN precursor-Hs00365052_m1 22.29 -3.85 0.97 IP10-Hs00171042_m1 31.75 -4.24 0.94
GAPDH-4342376-GAPDH 22.7 -3.55 0.98 Endothelin(EDN1)-Hs00174961_m1 31.77 -5.45 0.82
Stat3-Hs00234174_m1 24.03 -3.7 0.98 CD86-Hs00199349_m1 31.78 -5.3 0.93
HLA-DRA-Hs00219575_m1 25.37 -3.87 0.99 CD80-Hs00175478_m1 31.81 -5.14 0.86
CD54 (ICAM)-Hs00164932_m1 25.75 -3.74 0.99 TNF-a-Hs00174128_m1 31.84 -4.15 0.92
HO-1-Hs00157965_m1 26 -3.71 0.99 CD62E (SELP)-Hs00174583_m1 32.41 -5.07 0.96
GUSB-Hs99999908_m1 26.09 -3.67 0.97 CD25-Hs00166229_m1 32.44 -5.2 0.94
CD68-Hs00154355_m1 26.36 -4.02 0.99 ITAC-Hs00171138_m1 32.61 -5.3 0.94
VEGF A-Hs00173626_m1 26.63 -3.88 0.98 ACE-Hs00174179_m1 33.1 -4.33 0.82
TGF-b-Hs00171257_m1 26.78 -3.82 0.95 CCR5-Hs00152917_m1 33.77 -4.76 0.94
MCP-1-Hs00234140_m1 26.99 -3.85 0.98 IL-18-Hs00155517_m1 33.98 -4.25 0.88
BAX-Hs00180269_m1 27.51 -4.7 0.98 MEGALIN-Hs00189742_m1 34.1 -2.98 0.55
NFKB2-Hs00174517_m1 27.53 -3.87 0.98 COX-2-Hs00153133_m1 34.3 -3.35 0.86
CYP1A2-Hs00167927_m1 27.81 -3.71 0.99 CD154(TNFSF5)-Hs00163934_m1 34.57 -3.16 0.73
ECE-1-Hs00154837_m1 27.81 -3.81 0.97 IL-6-Hs00174131_m1 34.59 -4.09 0.93
Mip-1a-Hs00234142_m1 27.88 -3.74 0.99 FasL-Hs00181225_m1 34.74 -4 0.9
IkB2-Hs00395088_m1 28.24 -5.23 0.91 CCR4-NM_005508 34.99 -3.49 0.96
IL-1b-Hs00174097_m1 28.33 -5.27 0.9 CD34-Hs00156373_m1 35.3 -3.01 0.88
CD38-Hs00233552_m1 28.5 -3.41 0.98 CD28-Hs00174796_m1 35.53 -2.41 0.8
CD45-Hs00365634_g1 28.63 -4.16 0.98 AT1R(AGTR1)-Hs00241341_m1 35.53 -3.28 0.83
GNLY-Hs00246266_m1 28.74 -3.5 0.89 Collagen IV(COL4A5)-Hs00166712_m1 35.71 -3.2 0.82
CSF-1-Hs00174164_m1 28.86 -4.09 0.98 T-bet (TBX21)-Hs00203436_m1 35.75 -3.14 0.87
CD40-Hs00374176_m1 29.01 -3.49 0.98 CD19-Hs00174333_m1 36.35 -1.78 0.71
HLADR-NM_002124 29.11 -4.32 0.97 IL-12p35-Hs00168405_m1 36.57 -2.48 0.88
CD71-Hs99999911_m1 29.19 -3.7 0.98 IFN-g-Hs00174143_m1 36.84 -2.12 0.88
Rantes-Hs00174575_m1 29.19 -4.52 0.97 GZMB-Hs00188051_m1 36.99 -1.83 0.77
Mip-3c-Hs00171149_m1 29.43 -4.57 0.98 MADH-3-Hs00232219_m1 37.1 -2.21 0.79
Fas-Hs00163653_m1 29.5 -5.89 0.87 CYP7A1-Hs00167982_m1 37.17 -2.48 0.63
IL-7-Hs00174202_m1 29.74 -6.84 0.9 CD152-Hs00175480_m1 37.28 -1.46 0.64
SKI-Hs00161707_m1 30.31 -3.89 0.96 CXCR3-Hs00171041_m1 37.62 -1.24 0.49
MADH-7-Hs00178696_m1 30.48 -5.13 0.93 IL-15-Hs00174106_m1 37.92 -1.38 0.77
Beta Actin-Hs00174609_m1 30.57 -4.52 0.92 CCR7-Hs00171054_m1 38.15 -1.34 0.85
CD8-Hs00233520_m1 30.68 -4.61 0.99 iNos(Nos2A)-Hs00167248_m1 38.86 -0.54 0.43
PRF1-Hs00169473_m1 30.73 -4.22 0.9 IL-17-Hs00174383_m1 38.92 -0.51 0.43
BCL-XL-Hs00169141_m1 30.78 -3.37 0.99 TNF-b-Hs00236874_m1 38.97 -0.8 0.12
BCL2-Hs00153350_m1 31.22 -4.08 0.98 ICOS-Hs00359999_m1 38.98 -0.9 0.81
IL-10-Hs00174086_m1 31.33 -5.37 0.97 IL-5-Hs00174200_m1 38.98 -0.99 0.24
CD4-Hs00181217_m1 31.35 -4.93 0.97 IL-1a-Hs00174092_m1 39.39 -0.51 0.78
CD3-Hs00167894_m1 31.42 -5.68 0.93     
  1. This table shows data (from liver cDNA) generated from the 96 primer probe sets. There were quadruplicate determinations for each sample dilution. Mean Ct (copy threshold) of the undiluted liver cDNA is given for each gene. The slope and R2 values have been calculated. Genes with Ct values of 40 have not been included in the table.
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BMC Genomics

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