Overview of EST libraries from isolated immature Arabidopsis seeds. At the top, a representation of the available EST collections extracted from immature seeds. Lines in colour represent the period of development covered by the library. The library code according to the TIGR Arabidopsis Gene Index (http://www.tigr.org/tigr-scripts/tgi/T_index.cgi?species=arab ) is indicated next to the line. The number of ESTs available from the corresponding library is indicated above the line. Green lines correspond to previously existing EST collections, and the blue line corresponds to the new library described here. At the bottom, the stages of embryo and seed development, related to days after flowering (DAF), is shown . The main processes associated with seed development are indicated.