Figure 5

Panel A , Southern Blot analysis of TbDHN1. T. borchii genomic DNA digested with HindIII (H, Lane 1), KpnI (K, Lane 2) and SmaI (S, Lane 3) was probed with an ECL-labelled TbDHN1 cDNA probe. Panel B , Gel-blot analysis of the TbDHN1 transcript under salinity and low temperature stress. Induction (left): mRNA extracted from mycelia grown for 30 days on control medium (CM; Lane 1); treated for 30 min in NaCl 0.4 M (NaCl 30 min; Lane 2); treated for 24 h in NaCl 0.4 M (NaCl 24 h; Lane 3); treated for 48 h at 4°C (cold shock 48 h; Lane 4). Repression (right): mRNA extracted from mycelia grown for 30 days on CM (CM; Lane 1), treated for 29 h in NaCl 0.4 M (NaCl 29 h; Lane 2); treated for 24 h in NaCl 0.4 M and then transferred on CM for 5 h (NaCl 24 h→ CM 5h; Lane 3). Signals obtained by ³²P -labeled TbDHN1 probe (top) were normalized using the rRNA 28 S probe as internal standard (bottom).