Table 5 Comparison of zebrafish follicle protein repertoire deduced from SAGE with the protein repertoire isolated by proteomic analysis
SAGE-tag | Nbr* | GenBank | Cluster** | Gene | Protein name | UniProt accession number | Gel piece *** |
|---|---|---|---|---|---|---|---|
R1 or R1cr | |||||||
CATGTAAGACATCC | 226 | BC045879 | Dr.1109 | bactin2 | Beta-actin2 | Q7ZVI7, Q6NWJ6 | VII, 13 |
CATGTAAAACCAAA | 108 | AF057040 | Dr.25213 | bactin1 | Beta-actin1 | Â | Â |
CATGTTGATGAGGG | 113 | BC044190 | Dr.729 | ldhb | Lactate dehydrogenase B4 | Q9PVK4, Q803U5 | IX |
CATGCATTTTCTAA | 90 | BM571854 | Dr.19916 | zp2.3 | Egg envelope protein ZP2 variant B [zp2.3] | Q98SS2 | VII-IX, 10, 12–15 |
CATGCATTTTCTAA | 90 | BC093133 | Dr.23439 | zp2.4 | Zp2.4 protein | Q502R0 | VII-IX, 10, 12–15 |
CATGAGAAACGTTT | 77 | BC067692 | Dr.26977 | zp3b | Zona pellucida glycoprotein 3b | O12989, Q6NW78 | VI, 12 |
CATGGTGGTGTTGA | 75 | NM_131331 | Dr.30322 | zp3 | Zona pellucida glycoprotein 3 | Q9PWC7, Q6NW70 | V, VI, 8–12 |
CATGTTAATAAAAG | 53 | NM_198809 | Dr.5605 | tubb2 | Tubulin, beta, 2 | Q6TGT0, Q6P5M9, Q6IQJ2 | VI, 7 |
CATGTCTGTTTAAC | 47 | BC049058 | Dr.11308 | rplp0 | Ribosomal protein, large, P0 | Q7ZUG3, Q6P5K3 | VIII, IX |
CATGCTTAAATTGA | 40 | BC095386 | Dr.30628 | gapd | Gapdh protein (Fragment) | Q5XJ10 | VIII, IX |
CATGTTCTTTCTGT | 36 | BM778256 | Dr.23435 | zp2.2 | Egg envelope protein ZP2 variant A [zp2.2] | Q98SS3 | VII-IX, 10, 12–15 |
CATGCCACAGGGCA | 35 | CO917383 | Dr.5705 | rps3 | Ribosomal protein S3 | Q6TLG8 | IX |
CATGGCTGCCTTGG | 34 | NM_199795 | Dr.23436 | tuba4l | Tubulin alpha 3; Alpha tubulin-like protein | Q802Y6, Q6TGS5 | V, 7 |
CATGAGGATCGAGG | 30 | BI844143 | Dr.25678 | eno3 | Enolase 3, (beta, muscle) | Q568G3, Q6TH14 | VI, 9, 11, 12 |
CATGTACAAGGCTT | 28 | AY394968 | Dr.18315 | eef1g | Elongation factor 1-gamma | Q6PE25, Q6NYN8 | VI, 7, 8, 12 |
CATGTTCTCCCTGT | 25 | NM_001003844 | Dr.28231 | rpl6 | 60S ribosomal protein L6 | Q6DRC6, Q567N5 | X |
CATGTGCTGAAAGT | 16 | NM_213058 | Dr.26116 | hspa5 | Heat shock 70kDa protein 5 | Q7SZD3, Q6P3L3 | III |
CATGCCCGGTGGAT | 15 | BC045841 | Dr.24802 | hspa8 | Heat shock protein 8 | Q7ZVJ1, Q6TEQ5, Q6NYR4 | III |
CATGGTGTAAGTGA | 15 | NM_212772 | Dr.28850 | tuba8l | Tubulin, alpha 2 | Q6TNP9 | V, 7 |
CATGCTCAAAGATG | 12 | BG305824 | Dr.25868 | Â | Hypothetical protein zgc:64133 (L-lactate dehydro. activity) | Q7T334 | IX |
CATGTCTTCTGAAA | 12 | CO352070 | Dr.34146 | zp3al | Zona pellucida glycoprotein 3a, like | Q5TYP2 | V, VI, 9–12, 14, 15 |
CATGTCTTCTGAAA | 12 | CO351980 | Dr.25594 | zp3a | Zona pellucida glycoprotein 3a | Q5TYP5 | V, VI, 9–12, 14, 15 |
CATGTTTTTTTTGA | 11 | BC056719 | Dr.24206 | cct4 | Chaperonin containing TCP1, subunit 4 (Delta) | Q6PH46, Q6P123 | V, 8 |
CATGTCTTGGTTAA | 9 | NM_200153 | Dr.6969 | Â | Hypothetical protein zgc:55944 (aminopeptidase I activity) | Q803B5 | 8 |
CATGTGTTGATGAA | 8 | NM_199547 | Dr.3463 | ssb | Sjogren syndrome antigen B (Autoantigen La) | Q7ZTI0 | 12 |
CATGTGAAGCACTT | 8 | NM_212718 | Dr.18319 | Â | ZPA domain containing protein [si:dkeyp-50f7.2] | Q8JIX8, Q5TYX2 | III |
CATGAGAAGGAGGA | 6 | BC055516 | Dr.20938 | sod1 | Superoxide dismutase [Cu-Zn] | O73872 | 22 |
CATGTCTGAGCTGA | 4 | AY398308 | Dr.4751 | aldh2 | Mitochondrial aldehyde dehydrogenase 2 family [aldh2l] | Q6TH48, Q6NWJ9 | 10, 11 |
CATGAACCACTCAA | 4 | BC050953 | Dr.7644 | cct2 | Chaperonin containing TCP1, subunit 2 (Beta) | Q6PBW6 | V |
CATGACACAAAATA | 4 | BQ263267 | Dr.25009 | vg1 | Vitellogenin 1 | Q8JH36, Q8JH37, Q90YN8 | I-XI, 1–7, 10–17, 21–24 |
CATGTGCTCAAAAT | 4 | BC045933 | Dr.134 | cct7 | Chaperonin-containing T-complex protein 1 eta subunit | Q8JHG7 Q7ZTS3 | 7 |
CATGATTGTATGGT | 3 | BM860549 | Dr.7343 | Â | Acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain | Q7ZTH8, Q6NYM4, Q502R3 | VII, 12 |
CATGATGTGGATCC | 3 | CK673334 | Dr.28241 | rpl7a | Ribosomal protein L7a | Q6PBZ1 | X |
CATGCATTGTATAT | 3 | BM101604 | Dr.11543 | Â | Zgc:103482 | Q5XJA5 | VI, 12, 13 |
CATGCCGACCCTAC | 3 | BC083399 | Dr.11543 | Â | Zgc:103482 | Q5XJA5 | VI, 12, 13 |
CATGTTCTCCCTCA | 3 | BC091659 | Dr.10133 | Â | Zgc:110766 (translation elongation factor activity) | Q5BJ17 | 12, 13 |
CATGAAGATGGAAA | 3 | BQ285089 | Dr.24685 | Â | Hyp.prot.zgc:111961(hydro.-translo.F-type ATPase complex) | Q4VBK0 | VI |
CATGTTTTGTACAA | 2 | NM_200174 | Dr.8999 | Â | Hypothetical protein zgc:55702 | Q7ZVR7 | 8 |
CATGTCACAGGCCA | 2 | CO919203 | Dr.29735 | naca | Nascent polypeptide-associated complex alpha subunit | Q8JIU7 | IX |
CATGAAGCGGAGCT | 2 | BC059705 | Dr.28202 | Â | Hypothetical protein zgc:73404 (Peptidase) | Q6NSN3, Q6PBH6 | 10 |
CATGCAAGTGTGAT | 1 | BI843130 | Dr.20270 | Â | Wu:fi20c07 Hypothetical protein (Fragment) (Pentaxin) | Q7SZ53, Q5XJ77, Q4V8X3 | 17, 18 |
CATGCCTGATCTGT | 1 | NM_201052 | Dr.1065 | rpsa | Ribosomal protein SA | Q803F6 | 16, 20 |
CATGAGGGCTGGAA | 1 | NM_201290 | Dr.6928 | cct6a | Chaperonin containing TCP1, subunit 6A (Zeta 1) | Q7ZYX4 | IV, 7, 8 |
CATGTTGAACTCCA | 1 | BQ419723 | Dr.28864 | serpina1 | Novel protein similar to Alpha-1 antiproteinase (Serpina1) | Q5SPJ1, Q5SPJ4 | 10, 11 |
R2 or R3 | |||||||
CATGGAGATCTCTA | 2 | BC044432 | Dr.1051 | dldh | Dihydrolipoamide dehydrogenase | Q803L1, Q6TNU6 | 8 |
CATGTGTGCACGCA | 1 | BC095354 | Dr.39983 | Â | Hypothetical protein zgc:110655 | Q503F1 | IX |
Rn1 or Rn2 | |||||||
CATGTTAAAACGTG | 11 | CA471093 | Dr.7108 | hspd1 | Hspd1 protein (Heat shock 60 kD protein 1) | Q803B0 | IV |
CATGCTGAACGGAT | 5 | BC073176 | Dr.5529 | Â | GDP dissociation inhibitor 3;GDP dissociation inhibitor 2 | Q7ZVL9, Q6TNT9 | VI, 11 |
CATGCACATCAGAT | 1 | CK400766 | Dr.11310 | tuba1 | Tuba1 protein | Q8AWD6, Q6NWK7, O42271 | V, 7–9, 23 |
CATGATGAGACAAA | 1 | CO917509 | Dr.6501 | Â | Sb:cb825 protein (Fragment) | Q803D7 | V, 10 |
CATGCAAGTGTGTC | 1 | BC053271 | Dr.28298 | cct3 | Chaperonin-containing TCP-1 complex gamma chain | Q8JHI7, Q7T2P2 | IV |
R1 or R1cr, multiple matches | |||||||
CATGAAAAAAAAAA | 153 | BI879427 | Dr.296 | ckb | Creatine kinase, brain | Q7ZU04, Q8AY63, Q5RIR2 | VII, 12–15 |
CATGACATCACATT | 60 | AY394971 | Dr.664 | tuba8l4 | Similar to tubulin, alpha 1 (tubulin, alpha 8 like 4) | Q7ZVI6, Q6NWJ5 | V, 7–9, 23 |
CATGGCCATTGAGG | 4 | BI671108 | Dr.20576 | Â | Mitochondrial ATP synthase alpha subunit | Q6EE37 | V, 7 |
CATGAAGAAAAAAA | 3 | AF254638 | Dr.31985 | vg3 | Vitellogenin 3 (Fragment) [vg3] | Q9DFT9 | II, VIII, 6, 21 |
CATGATCACTGGAA | 3 | BM182705 | Dr.15108 | Â | LOC407641 protein (Fragment) translation elongation factor | Q7ZWA1 | VI |
CATGTATAAATTCC | 2 | NM_001002374 | Dr.31390 | Â | Zgc:92082 | Q6DHT4 | 8, 9 |
CATGTGTGTGTGAG | 1 | BG304242 | Dr.7952 | pkm2 | Pyruvate kinase, muscle | Q7ZVT2, Q6NXD1 | 7, 8, 9 |
CATGCAGGAGTTCA | 1 | CA496254 | Dr.4724 | eno1 | Enolase 1, (Alpha) [zgc:73152] | Q6IQP5 | 8, 9, 11, 12 |
Non detected | |||||||
CATGTGGCTTTCAA | 0 | NM_200446 | Dr.831 | chia | Eosinophil chemotactic cytokine | Q7SYA8, Q6TH31 | IV |
CATGCTGGACCAGC | 0 | NM_131804 | Dr.10788 | nots | Nots protein | Q5PR42, Q9DDE2 | 16, 20 |