Molecular phenotype of zebrafish ovarian follicle by serial analysis of gene expression and proteomic profiling, and comparison with the transcriptomes of other animals

Table 6 Comparison of zebrafish follicle transcript occurrence of proteins identified by proteomic analysis, with the corresponding protein repertoire deduced from transcripts expressed at over 0.15% of the mRNA population in human ovary cDNA libraries

Zebrafish*	Human**	Human gene	Protein name	Zebrafish			Human
				SAGE	ID.9767	ID.15519	ID.4908	ID.5611	ID.10552
Dr.1109	Hs.520640	ACTB	Beta-actin	0.822	0.317	0.752	0.404	0.503	0.535
Dr.25213	Hs.520640	ACTB	Beta-actin	0.393	0.326	0.698	0.404	0.503	0.535
Dr.729	Hs.446149	LDHB	Lactate dehydrogenase B	0.411	0.035	0.046	0.210	0.140	0.197
Dr.5605	Hs.433615	TUBB2	Tubulin, beta, 2	0.193	0.290	0.852	0.194	0.140	0.159
Dr.11308	Hs.448226	RPLP0	Ribosomal protein, large, P0	0.171	0.079	0.023	0.381	0.199	0.384
Dr.30628	Hs.544577	GAPDH	Glyceraldehyde-3-phos. dehydro.	0.145	0.096	0.116	1.105	1.018	0.816
Dr.5705	Hs.546286	RPS3	Ribosomal protein S3	0.127	0.017	0.008	0.708	0.889	0.356
0Dr.18315	Hs.444467	EEF1G	Elongation factor 1-gamma	0.101	0.052	0.089	0.700	0.304	0.525
Dr.28241	Hs.558380	RPL7A	Ribosomal protein L7a	0.011	0.088	0.004	0.591	0.081	0.366
Dr.1065	Hs.558354	RPSA	Ribosomal protein SA	0.003	0.088	0.008	0.342	0.187	0.103
Dr.7952	Hs.198281	PKM2	Pyruvate kinase, muscle	0.003	0	0.017	1.035	0.374	0.657
Dr.4724	Hs.517145	ENO1	Enolase 1	0.003	0	0	2.023	0.573	0.582

*Zebrafish UniGene Build#87. **Human UniGene Build#187. The values indicated are the percentages of species specific UniGene clusters as evaluated by the percentages of corresponding tags or EST counts in the mRNA population identified in ZEBRAOV SAGE tag database and dbEST libraries. Percentages at over 0.15% were bolded.

ISSN: 1471-2164