Identification of rat lung – prominent genes by a parallel DNA microarray hybridization

BMC Genomics

Table 1 Slide layout and hybridization design

Slide	Block A		Block B		Block C
	Green	Red	Green	Red	Green	Red
1	Lung	Heart	Liver	Brain	Kidney	Spleen
2	Heart	Kidney	Liver	Lung	Spleen	Brain
3	Kidney	Liver	Lung	Brain	Spleen	Heart
4	Heart	Brain	Kidney	Lung	Liver	Spleen
5	Heart	Liver	Lung	Spleen	Brain	Kidney

The array has three identical blocks, A, B, and C; each containing 9,984 spots representing 6,221 known rat genes, 3,594 ESTs, and 169 Arabidopsis negative controls. The three blocks are separated with thermal plastic rings. Three paired Cy3-(green) and Alexa 647 (red)-labeled cDNA samples were hybridized onto three blocks, A, B, and C of slide 1–5. Dye and sample assignments are random for each slide. Five slides represent technical replications.

ISSN: 1471-2164