Analysis of the endonuclease family of proteins. (A) Multiple sequence analysis of salivary endonucleases from Phlebotomus and Lutzomyia sandflies. Sequences were aligned using ClustalX and manually refined using BioEdit sequence-editing software. (B) Phylogenetic tree analysis of endonucleases from sandflies and other organisms, including S. thypimurium, Anabaena sp., F. tularensis, H. pylori, S. serevisiae, M. musculus, H. sapiens, P. phaeoclathratiform, X. oryzar, Polaromonas sp., R. solanacearum, R. metallidurans, C. pippiens, G. morsitans, D. melanogaster, P. cantschaticus and M. japonicus. Phylogenetic analysis was conducted on protein alignments using Tree Puzzle version 5.2 by maximum likelihood using quartet puzzling, automatically estimating internal branch node support (1000 replications).