Analysis of apyrase protein family. (A) Multiple sequence analysis of apyrases from the salivary glands of Phlebotomus argentipes (Pag), Phlebotomus ariasi (Par) and Phlebotomus perniciosus (Ppe). Sequences were aligned using ClustalX and manually refined using BioEdit sequence-editing software. (B) Phylogenetic tree analysis of apyrase protein family from P. argentipes (Pag), P. ariasi (Par), P. perniciosus (Ppe), L. longipalpis (LJ), P. papatasi, Cimex lectularius, and transcripts coding from this protein family (identified at GenBank) from Drosophila melanogaster, An. Gambiae, A. mellifera, C. elegans, M. musculus, R. ratus, B. Taurus, H. sapiens, P. troglodytes, G. galus, D. rerio and X. leavis. Phylogenetic analysis was conducted on protein alignments using Tree Puzzle version 5.2 by maximum likelihood using quartet puzzling, automatically estimating internal branch node support (1000 replications).