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Figure 3 | BMC Genomics

Figure 3

From: "Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays

Figure 3

Positioning of GSC spike mRNAs in Affymetrix GeneChip dose-response range. A frequency histogram of the probe sets of Affymetrix GeneChip Mouse430-2 is shown. The histogram for all probe sets (gray) shows near-normal distribution. Blue columns are the "present" calls (P), red columns "absent" calls (A) and green "marginal" calls. The five yellow lines indicate the positions of the GSC spike mRNAs that are chosen to cover the "present" call range by a proper "spike factor".

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