Figure 4
From: "Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays
The dose-response linearity of the GSC spikes in Q-PCR and the Affymetrix GeneChip array system. Linear relationships are shown between (a) the Q-PCR Ct values and log of copy number (log (C')), and (b) the GeneChip log signal intensity (log(S)) and log of copy number (log (C')) of the GSC mRNAs. The regression functions were obtained by the least squares method. The inverse functions (*) were further used to generate the copy numbers of all other genes/probe sets for Percellome normalization.