Figure 6

Uterotrophic response of ovariectomized female mice by an estrogenic test compound.(a) Shows the uterine weight, which increases in a dose-dependent manner; V, vehicle control; Low, low dose; ML, medium-low dose; MH, medium-high dose; High, high dose group. (b) Shows the line display of uterine gene expression (Affymetrix MG-U74v2 A GeneChips) normalized by global normalization (90 percentile), and (c) by the Percellome normalization. Averages of three samples per group were visualized (by K. A.). The five white lines are the GSC mRNAs. The green and blue lines are actin (AFFX-b-ActinMur/M12481_3_at) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase, AFFX- GapdhMur/M32599_3_at), respectively. By global normalization, 7,400 probe sets remained unchanged and 4,600 probe sets increased more than two-fold in the H group compared to the V group, whereas almost all probe sets measured had increased. It is noted that housekeeping genes such as actin and GAPDH are significantly induced on a per cell basis.