Figure 7

Conversion functions between Q-PCR and GeneChip. The data shown in Figure 5 as 3D surfaces are shown as a scatter plot (60 plots). The regression function can be used to convert Q-PCR to GeneChip and vice versa, with a level of certainty indicated by coefficient of correlation. It is noted that Cyp1a1 and Cyp1a2 became saturated above about 400 copies per cell in GeneChip system (indicated in pink plots). Cyp7a1 showed high linearity, indicating that the variation shown by the split +1sd and -1sd surfaces in Figure 5 reflected biological (animal) variation, not measurement errors.