Table 1 The spike factors for various organs/tissues
From: "Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays
Species | Organ/Tissue (adult, unless otherwise noted) | Spike Factor | total RNA/genomic DNA | SD |
|---|---|---|---|---|
Mouse | Liver | 0.2 | 211 | 46 |
Mouse | Lung | 0.02 | 22 | 4 |
Mouse | Heart | 0.05 | - | - |
Mouse | Thymus | 0.01 | 8 | 2 |
Mouse | Colon Epitherium | 0.05 | 105 | 30 |
Mouse | Kidney | 0.1 | - | - |
Mouse | Brain | 0.1 | - | - |
Mouse | Suprachiasmatic nucleus (SCN) | 0.1 | - | - |
Mouse | Hypothalamus | 0.1 | 63 | 4 |
Mouse | Pituitary | 0.1 | 52 | 8 |
Mouse | Ovary | 0.02 | 35 | 4 |
Mouse | Uterus | 0.02 | 42 | 12 |
Mouse | Vagina | 0.02 | 81 | 38 |
Mouse | Testis | 0.15 | 56 | 7 |
Mouse | Epididymis | 0.07 | 53 | 16 |
Mouse | Bone marrow | 0.02 | 14 | 3 |
Mouse | Spleen | 0.02 | - | - |
Mouse | Whole Embryo | 0.15 | 97 | 36 |
Mouse | Fetal Telencephalon E10.5–16.5 | 0.1 | 48 | 9 |
Mouse | Neurosphere (E11.5–14.5) | 0.03 | 42 | 10 |
Mouse | E9.5 embryo heart | 0.15 | 58 | 15 |
Mouse | cell lines | 0.2 | - | - |
Rat | Liver | 0.2 | - | - |
Rat | Kidney | 0.2 | - | - |
Rat | Uterus | 0.04 | 56 | 5 |
Rat | Ovary | 0.04 | 56 | 9 |
Human | Cancer Cell Lines | 0.2 | 116 | 26 |
Xenopus | liver | 0.03 | - | - |
Xenopus | embryo | 0.15 | - | - |