Figure 3
Mechanism of recombination between mutant loxP spacers. Input lox 71 left inverted repeat mutant (LE) and lox 66 right inverted repeat mutant (RE) oligonucleotide sequences with scissile phosphodiester bonds highlighted with blue arrows. Cre-mediated recombination between the LE mutant and RE mutant resulted in one loxP site containing both mutants and another with wild-type inverted repeats. The input LE/RE spacer mutants of the reacting oligonucleotides were inferred by working backwards from the location of the scissile bonds in the final sequenced recombinant.