Table 2 Oligonucleotide sequences (LE, RE) and primers used in the high-throughput LE/RE-spacer mutant loxP screen
Name | Sequence |
|---|---|
LE | 5' < CTGAGCTCGGGTGCCTGTTACCGTTCGTATANNNTANNNTATACGAAGTTATGACTGCACCCATCAGAGC > 3' |
LE_EcoRI_Forward | 5' < TGACGAATTCCTGAGCTCGGGTGCCTGT > 3' |
LE_HindIII_Reverse | 5' < GCTAAAGCTTGCTCTGATGGGTGCAGTC > 3' |
RE | 5' < TGTCTCTGGCACGCTAGGATAACTTCGTATANNNTANNNTATACGAACGGTATATGGCCTTCTGTAGCGG > 3' |
RE_NotI_Forward | 5' < GTACGCGGCCGCTGTCTCTGGCACGCTAGG > 3' |
RE_NotI_Reverse | 5' < GTACGCGGCCGCCCGCTACAGAAGGCCATA > 3' |