Figure 1

Characterization of the DoxHPC lines. A. Real-time PCR analyses of Lhx2 expression in three independent DoxHPC lines at the indicated time points after dox withdrawal. Relative Lhx2 expression is compared to the expression in each DoxHPC lines in the presence of dox (Day 0) and this expression is arbitrarily set as 1. B. Flow cytometry analysis of GFP expression at the indicated time points after dox withdrawal in the DoxHPC line where GFP expression is linked to Lhx2 expression by an ires. C. Cobblestone areas formed after seeding DoxHPC lines onto S17 stromal cells (upper panel) and maintenance of blast-like cells in these cultures (lower panel) in the presence of dox. D. Lack of cobblestone areas after seeding DoxHPC lines onto S17 stromal cells (upper panel) and differentiation into macrophages (lower panel) in the absence of dox. E. May-Grünwald Giemsa staining of cytospun cells from a DoxHPC line cultured in the presence of dox (Day 0) as compared to different time points after dox withdrawal.