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Table 1 Bacterical strains and plasmids

From: Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1

Strain or plasmid

Relevant characteristics

Source or reference

E. coli

  

   S17-1/λpir

RK2 tra regulon, pir, host for pir-dependent plasmids

26

   WG1

S17-1/λpir strain harboring suicide plasmid pWG3

This study

S. oneidensis

  

   MR-1

Wild-type strain

2

   DSP-10

Spontaneous rif ampin-resistant derivative of the MR-1, used as the reference strain in replace of MR-1 in this study

11

   WG2

Transconjugant between E. coli WG1 and S. oneidensis DSP-10, containing a chromosomal integration of suicide plasmid pWG3

This study

   WG3

In-frame S01377 gene deletion mutant of 1496-bp, derivative from WG2

This study

Plasmids

  

   pCVD422

Suicide plasmid, bla oriT oriR6K sacB

27

   pBSL142

pBluescript derivative, containing gentamicin -resistance gene

28

   pDC3.O

Suicide plasmid generated by cloning the gentamicin-resistance gene from pBSL142 into the EcoRV site of pCV442

This study

   pWG3

Suicide plasmid generated by cloning a 1.1 kb DNA fragment containing the mutated S01377 gene with 1496-bp deletion into the Sad site of pDS3.0.

This study

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BMC Genomics

ISSN: 1471-2164

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