Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1

Table 4 Identification of protiens showing signifinant difference in abundance on 2-D PAGE using MIcro-LC-ESI-MS/MS

Spot No.	Gene	Symbol	Putative function	Volume in wild type	Volume in mutant
202	SO1637		bacterial surface antigen	21077 (2592)	10347 (2287)
333	SO1377		conserved hypothetical protein	49308 (5917)	0
422	SO1117		putative cytosol aminopeptidase	21725 (1716)	10017 (771)
426	SO2407		conserved hypothetical protein	71070 (11798)	120011 (11641)
433	SO2767	asnB-1	asparagine synthetase B, glutamine-hydrolyzing	49614 (5917)	0
482	SO4749	atpA	ATP synthase F1, alpha subunit	25802 (4670)	12153 (4837)
748	SO3505	nagA	N-acetylglucosamine-6-phosphate deacetylase	22356 (4561)	37349 (2876)
1160	SO3466	ribH	riboflavin synthase, beta subunit	43043 (9986)	15730 (3665)
235	ND		ND	12369 (2845)	32860 (6604)
663	ND		ND	19704 (3527)	9644 (1948)
741	ND		ND	11690 (2923)	35919 (4382)

Expression changes are considered as signifinant(<0.001 probability in Stident's t-test). Number in parenthesis indicate standard deniation. spot volume is a relative measure of integrated density

ISSN: 1471-2164