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Table 1 Classification of common differentially regulated temporal gene expression responses to TCDD in both in vitro and in vivo models

From: In vivo – in vitro toxicogenomic comparison of TCDD-elicited gene expression in Hepa1c1c7 mouse hepatoma cells and C57BL/6 hepatic tissue

Accession Gene name Gene symbol Entrez Gene ID In vivo In vitro
     Fold changea Time pointsb EC50c,d (μg/kg) Fold changea Time pointsb EC50c,d (pM)
I) Induced both in vivo and in vitroe
BE689910 RIKEN cDNA 2310001H12 gene 2310001H12Rik 69504 2.7 2f, 168 48.02 3.9 1f ND
BF226070 RIKEN cDNA 2600005C20 gene 2600005C20Rik 72462 2.1 4, 12, 18, 24f, 72, 168 2.18 2.3 4, 8, 12f, 24, 48 265.50
AI043124 RIKEN cDNA 2810003C17 gene 2810003C17Rik 108897 1.6 12f 37.02 1.7 4f ND
AW537038 expressed sequence AA959742 AA959742 98238 7.2 4, 8, 12f, 18, 24, 72, 168 1.71 5.2 4, 8f, 12, 24, 48 67.79
W34507 abhydrolase domain containing 6 Abhd6 66082 1.7 4, 8f, 12, 18, 24, 72, 168 154.30 1.5 48f 138.50
NM_026410 cell division cycle associated 5 Cdca5 67849 8.8 4, 8, 12, 18, 24, 72f, 168 ND 1.7 4f ND
BG063743 craniofacial development protein 1 Cfdp1 23837 3.6 4, 8, 12f, 18, 24, 72, 168 14.27 2.3 4f, 8, 12, 24, 48 42.64
AA073604 procollagen, type I, alpha 1 Col1a1 12842 1.7 18, 24, 72f 0.65 1.6 4, 8, 12f 17.25
NM_009992 cytochrome P450, family 1, subfamily a, polypeptide 1 Cyp1a1 13076 38.4 2, 4, 8, 12, 18, 24f, 72, 168 0.05 37.7 1, 2, 4, 8, 12, 24, 48f 14.06
BE457542 Dehydrogenase/reductase (SDR family) member 3 Dhrs3 20148 2.0 4, 8, 12f, 18, 72, 168 0.67 1.5 8f 2.43
AW552715 DnaJ (Hsp40) homolog, subfamily B, member 11 Dnajb11 67838 1.7 12, 18, 24f,168 3.95 1.6 8, 12f 9.85
AK015223 dermatan sulphate proteoglycan 3 Dspg3 13516 6.2 4, 8, 12, 18, 24f, 72, 168 0.13 8.4 2, 4, 8, 12, 24, 48f 16.34
NM_008655 growth arrest and DNA-damage-inducible 45 beta Gadd45b 17873 4.6 2f, 4, 72 133.30 3.7 1f, 2 1440.00
W54349 glutathione S-transferase, alpha 4 Gsta4 14860 2.0 18, 24, 72f 0.48 1.7 8f, 12 56.38
BG067127 interferon regulatory factor 1 Irf1 16362 1.5 168f ND 1.7 2f, 4 ND
AA015278 integrin beta 1 (fibronectin receptor beta) Itgb1 16412 1.6 4, 18, 24, 168f 97.23 4.2 4, 8, 12, 24, 48f 72.92
AA041752 Jun proto-oncogene related gene d1 Jund1 16478 2.0 12f, 18, 24 0.99 2.1 4, 8, 12, 24, 48f 50.34
BF538945 lectin, mannose-binding, 1 Lman1 70361 1.9 12, 72, 168f 13.49 2.0 4f, 8, 24, 48 40.72
BG066626 lipin 2 Lpin2 64898 3.0 4, 12, 24f, 72 3.13 2.3 2, 4f, 8, 12, 24, 48 23.83
BI440950 leucine rich repeat containing 39 Lrrc39 109245 2.9 2f, 4 49.71 3.1 1f, 2 68.57
AW413953 mitochondrial ribosomal protein L37 Mrpl37 56280 8.3 2, 4, 8f, 12, 18, 24, 72, 168 8.77 2.7 2, 4f, 8, 12, 24, 48 49.59
BE623489 NAD(P)H dehydrogenase, quinone 1 Nqo1 18104 4.6 4, 8, 12f, 18, 24, 72, 168 1.00 5.2 4, 8f, 12, 24, 48 33.74
NM_026550 PAK1 interacting protein 1 Pak1ip1 68083 3.8 4, 8, 12, 18, 24, 72f, 168 0.26 2.2 4f, 8, 12, 24, 48 7.00
AA152754 prostaglandin-endoperoxide synthase 1 Ptgs1 19224 1.6 168f 1.11 2.3 4, 8f, 12, 24, 48 37.96
BG063583 solute carrier family 20, member 1 Slc20a1 20515 2.2 2, 4f, 8 ND 1.8 2f, 4 ND
AJ223958 solute carrier family 27 (fatty acid transporter), member 2 Slc27a2 26458 1.9 12f, 18, 24, 72, 168 2.88 2.1 8, 12f, 24, 48 17.42
BG066820 solute carrier family 6 (neurotransmitter transporter, taurine), member 6 Slc6a6 21366 1.8 4f, 12 2.48 1.7 48f 3.06
AI592773 suppression of tumorigenicity 5 St5 76954 1.6 8, 12f 28.85 1.7 4f, 8, 12 14.69
BG067168 TCDD-inducible poly(ADP-ribose) Polymerase Tiparp 99929 10.3 2, 4f, 12, 18, 24, 72, 168 36.49 6.4 1, 2f, 4, 8, 12, 24, 48 18.03
BG065761 tumor necrosis factor, alpha-induced protein 2 Tnfaip2 21928 5.5 2, 4f, 12, 18, 72 36.41 6.3 2, 4f, 8, 12, 24, 48 41.15
AA067191 UDP-glucose dehydrogenase Ugdh 22235 3.1 4, 8, 12 f, 18, 24, 72, 168 0.79 1.5 2, 4, 8, 12f, 48 4.33
NM_011709 whey acidic protein Wap 22373 5.9 2, 4, 8, 12, 18, 24, 72, 168f 0.12 4.2 2, 4, 8, 12, 24, 48f 17.44
BG075778 Xanthine dehydrogenase Xdh 22436 2.7 4, 8, 12f, 18, 24, 72, 168 1.24 2.6 4, 8, 12, 24, 48f 34.92
BG073881 zinc finger protein 36, C3H type-like 1 Zfp36l1 12192 2.2 2f ND 1.7 1, 2f 2427.00
AA031146 zinc finger protein 672 Zfp672 319475 1.6 4f 3.09 1.5 2f ND
II) Repressed both in vivo and in vitroe
BG146493 RIKEN cDNA 6330406L22 gene 6330406L22Rik 70719 -1.5 18f 0.51 -1.8 8, 12f 25.67
AA140059 DNA methyltransferase (cytosine-5) 1 Dnmt1 13433 -1.9 168f ND -1.6 8f, 12 ND
AI327022 ficolin A Fcna 14133 -1.6 18, 24f ND -1.7 12, 24f ND
AA288963 fibrinogen-like protein 1 Fgl1 234199 -1.9 24f ND -1.5 24f, 48 ND
BE626913 GTP binding protein 6 (putative) Gtpbp6 107999 -3.4 24, 72f ND -1.7 24, 48f 116.40
AA275564 glycerol kinase Gyk 14933 -1.5 12f 10.2 -1.6 24f ND
BG070106 lipocalin 2 Lcn2 16819 -2.8 24f ND -1.5 24, 48f ND
AW049427 leucine zipper domain protein Lzf 66049 -1.6 24f ND -1.6 48f 78.29
AA016759 minichromosome maintenance deficient 6 Mcm6 17219 -1.6 18f 3.34 -1.6 8f 58.04
BF011268 mitochondrial methionyl-tRNA formyltransferase Mtfmt 69606 -1.8 24, 72f, 168 ND -1.6 24, 48f ND
AA683699 RNA (guanine-7-) methyltransferase Rnmt 67897 -2.0 12f ND -1.6 8f ND
  syntrophin, gamma 1 Sntg1 71096 -1.6 24f 15.27 -1.7 4f 66.61
AA199550 syntaxin 12 Stx12 100226 -1.5 18f ND -1.6 48f ND
AA047942 thymidine kinase 1 Tk1 21877 -1.7 18f, 24, 72 0.34 -2.0 8, 12f 153.90
III) Induced in vivo and repressed in vitroe
AA122925 carbonic anhydrase 2 Car2 12349 2.4 12, 72, 168f 2.00 -1.8 24f, 48 55.96
AI327078 coactosin-like 1 Cotl1 72042 1.6 168f ND -1.7 24, 48f 25.19
NM_007935 enhancer of polycomb homolog 1 Epc1 13831 1.6 168f 1.16 -2.7 12, 24f 75.21
BC002008 fatty acid binding protein 5, epidermal Fabp5 16592 3.9 8, 12f 2.43 -1.9 8, 12f, 24 54.14
NM_026320 growth arrest and DNA-damage- inducible, gamma interacting protein 1 Gadd45gip1 102060 1.8 168f 4.67 -1.5 8f 40.49
W11419 inhibitor of DNA binding 3 Id3 15903 1.8 168f 0.34 -1.5 24, 48f 88.83
AA009268 myelocytomatosis oncogene Myc 17869 3.7 4, 12f, 168 5.59 -2.2 2f 148.40
NM_011033 poly A binding protein, cytoplasmic 2 Pabpc2 18459 7.0 2f ND -1.6 12f ND
  REST corepressor 1 Rcor1 217864 1.9 4, 8, 18, 72, 168 3.70 -1.6 24f 116.50
BE980584 secretory granule neuroendocrine protein 1, 7B2 protein Sgne1 20394 3.3 168f 0.74 -1.5 48f 175.00
AA462951 transcription factor 4 Tcf4 21413 1.6 12f, 168 5.77 -1.5 24f 74.44
AA003942 tenascin C Tnc 21923 1.6 168f 0.37 -1.8 24f, 48 59.34
IV) Repressed in vivo and induced in vitroe
W36712 B-cell translocation gene 2, anti- proliferative Btg2 12227 -1.8 18f, 24 ND 1.5 4f ND
AA174215 cathepsin L Ctsl 13039 -1.6 24f, 72, 168 ND 1.6 8, 48f ND
AA419858 cysteine rich protein 61 Cyr61 16007 -1.6 2f 0.07 1.6 8, 48f ND
AW488956 polo-like kinase 3 Plk3 12795 -1.6 4f ND 1.6 4, 48f ND
BG068288 solute carrier organic anion transporter family, member 1b2 Slco1b2 28253 -1.7 8f, 12, 18, 24, 72, 168 ND 1.6 4f 1.18
NM_011470 small proline-rich protein 2D Sprr2d 20758 -1.6 18f, 72 1.97 1.6 4f ND
  1. aMaximum absolute fold change determined by microarray analysis
  2. bTime point where genes are differentially regulated with P1(t) > 0.9999 and |fold change| > 1.5
  3. cEC50 valued determined from microarray results
  4. dND = not determined from microarray results
  5. eClassification groups as defined in Figure 4B
  6. fTime point representing the maximum |fold change|