Figure 2

Amino acid identity in alignments of constitutive and alternative coding regions of 3029 alternatively spliced genes classified by the overall evolution rate. C – constitutive regions. A – alternative regions. AN – N-terminal alternative regions. AI – internal alternative regions. AC – C-terminal alternative regions. Five columns on the left show results for three equal bins of slowly evolving genes, genes evolving with medium speed, and fast evolving genes. Five columns on the right correspond to the total sample of 3029 alternatively spliced genes. To estimate the average amino acid identity, we used global meta-alignments (see Methods). The boxplots represent the results for 2000 bootstrap replications.