Table 1 Primer sequences and conditions for RT-PCR. Gene sequences used for primer design were retrieved from the Ensembl website [36], annealing temperature and MgCl₂ concentration used in PCR reactions are listed.

KRT6A

ENSG00000074729

5'-CTG AGG CTG AGT CCT GGT AC-3'

5'-GTT CTT GGC ATC CTT GAG G-3'

56

2.5

SPRR1A

ENSG00000169474

5'-ACA CAG CCC ATT CTG CTC CG-3'

5'-TGC AAA GGA GCG ATT ATG ATT-3'

52

2

SPRR1B

ENSG00000169469

5'-AGA CCA AGC AGA AGT AAT GTG-3'

5'-AGA CCT TCA GCT TCA TTC AGA G-3'

61

4

SPRR2A

ENSG00000163212

5'-TGG TAC CTG AGC ACT GAT CTG CC-3'

5'-CCA AAT ATC CTT ATC CTT TCT TGG-3'

58

2

IL1F9

ENSG00000136688

5'-TGG GAA TCC AGA ATC CAG-3'

5'-TTG GCA CGG TAG AAA AGG-3'

61

3.5

S100A2

ENSG00000160675

5'-CAA GAG GGC GAC AAG TTC-3'

5'-GCC CAT CAG CTT CTT CAG-3'

59

3

CALML5

ENSG00000178372

5'-GGT TGA CAC GGA TGG AAA CG-3'

5'-AAC CTC GGA GAT GAG TTT CCT TAG-3'

60

3

ACTB

ENSG00000075624

5'-AAG GAA GGC TGG AAG AGT GC -3'

5'-CTA CAA TGA GCT GCG TGT GG -3'

56

2