Figure 2

Multiple sequence alignment of 61 selected representatives of the GIY-YIG superfamily. Sequences were selected from each family (UvrC, Cho, Cho+Exo, Cho-like, Cho-like+Exo, Bacillus-1, Bacillus-2, HEases, REases, Penelope, COG3680, COG1833, Slx, MutS-like) to cover diversity of known structures and functions. Sequences are denoted by the species' name, the NCBI gene identification (GI) number and the PDB code (if applicable). Additionally sequences are grouped by families listed above. The variable termini and insertions are not shown; the number of omitted residues is indicated in parentheses. Amino acids are colored according to the physico-chemical properties of their side-chains (negatively charged: red, positively charged: blue, polar: magenta, hydrophobic: green). Conserved residues are highlighted. Secondary structure elements determined for the archaetypal member of the superfamily, I-TevI, are shown as H (helices) and E (strands).