Definition of CDF clusters and substrate specificities. The characterised members along with their major substrate specificities, the corresponding references and histidine-rich (or serine/cysteine-rich) regions are indicated for each cluster. When more than one metal is transported, the metal preference is reflected by the written order within these metals. For each group and for Zrg17-like and ZnT9-like clusters logos showing the conserved residues in TMDs II and V are shown on the right. A simplified phylogenetic tree representation, expressed as a dendogram using Zrg17-like cluster as outgroup, is shown on the left. 1 B. Montanini, D. Blaudez, M. Chalot, unpublished data; 2 D. Blaudez, M. Chalot, unpublished data; 3 assessed by heterologous complementation; 4 deduced by mutant phenotype or over-expression in homologous system; 5 measured in reconstituted proteoliposomes or in everted membrane vesicles; 6 indirect evidence; 7 measured in Xenopus laevis oocytes; 8 by referring to the CDF domain.