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Table 2 Oligonucleotides used for the introduction of amino acid substitutions in PtdMTP1

From: Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity

Primer name Position Sequence Substitution
C30Sf 72-102 TGGAGGAAGCAAGGGAaGCGGGGAAGCACCT C30S
C30Sr 102-72 AGGTGCTTCCCCGCtTCCCTTGCTTCCTCCA C30S
C35Sf 87-118 TGCGGGGAAGCACCTTgTGGATTTTCAGAT C35S
C35Sr 118-87 ATCTGAAAATCCAcAAGGTGCTTCCCCGCA C35S
C64Sf 175-205 ATATCCGTGGCACTTTcTATAGTCTTCATG C64S
C64Sr 205-175 CATGAAGACTATAgAAAGTGCCACGGATAT C64S
G118Af 343-372 CAATCTTATGcATTTTTTAGGATTGAGATT G118A
G118Ar 372-343 AATCTCAATCCTAAAAAATgCATAAGATTG G118A
H260Df 764-793 AAGGAGCTTATCTCgATGTACTTGGGGATT H260D
H260Dr 793-764 AATCCCCAAGTACATcGAGATAAGCTCCTT H260D
D264Ef 775-804 CTCCATGTACTTGGGGAaTCCATCCAGAGT D264E
D264Er 804-775 ACTCTGGATGGAtTCCCCAAGTACATGGAG D264E
D264Af 775-804 CTCCATGTACTTGGGGcTTCCATCCAGAGT D264A
D264Ar 804-775 ACTCTGGATGGAAgCCCCAAGTACATGGAG D264A
D288Ef 847-876 GAGTGGAAGATAATTGAaCTGATCTGCACC D288Ef
D288Er 876-847 GGTGCAGATCAGtTCAATTATCTTCCACTC D288Ef
D288Af 847-876 GAGTGGAAGATAATTGcTCTGATCTGCACC D288A
D288Ar 876-847 GGTGCAGATCAGAgCAATTATCTTCCACTC D288A
C291Sf 855-885 ATAATTGATCTGATCTcCACCCTAATCTTT C291S
C291Sr 885-855 AAAGATTAGGGTGgAGATCAGATCAATTAT C291S
C357Sf 1053-1083 AAGATTCTCTTGGCTTcTCATGTGAAAATC C357S
C357Sr 1083-1053 GATTTTCACATGAgAAGCCAAGAGAATCTT C357S
  1. For each oligonucleotide the position on the PtdMTP1 coding sequence is shown, together with the sequence and the target mutation. Nucleotides written in lower case were mutated with respect to the original sequence.