Table 2 Oligonucleotides used for the introduction of amino acid substitutions in PtdMTP1
Primer name | Position | Sequence | Substitution |
|---|---|---|---|
C30Sf | 72-102 | TGGAGGAAGCAAGGGAaGCGGGGAAGCACCT | C30S |
C30Sr | 102-72 | AGGTGCTTCCCCGCtTCCCTTGCTTCCTCCA | C30S |
C35Sf | 87-118 | TGCGGGGAAGCACCTTgTGGATTTTCAGAT | C35S |
C35Sr | 118-87 | ATCTGAAAATCCAcAAGGTGCTTCCCCGCA | C35S |
C64Sf | 175-205 | ATATCCGTGGCACTTTcTATAGTCTTCATG | C64S |
C64Sr | 205-175 | CATGAAGACTATAgAAAGTGCCACGGATAT | C64S |
G118Af | 343-372 | CAATCTTATGcATTTTTTAGGATTGAGATT | G118A |
G118Ar | 372-343 | AATCTCAATCCTAAAAAATgCATAAGATTG | G118A |
H260Df | 764-793 | AAGGAGCTTATCTCgATGTACTTGGGGATT | H260D |
H260Dr | 793-764 | AATCCCCAAGTACATcGAGATAAGCTCCTT | H260D |
D264Ef | 775-804 | CTCCATGTACTTGGGGAaTCCATCCAGAGT | D264E |
D264Er | 804-775 | ACTCTGGATGGAtTCCCCAAGTACATGGAG | D264E |
D264Af | 775-804 | CTCCATGTACTTGGGGcTTCCATCCAGAGT | D264A |
D264Ar | 804-775 | ACTCTGGATGGAAgCCCCAAGTACATGGAG | D264A |
D288Ef | 847-876 | GAGTGGAAGATAATTGAaCTGATCTGCACC | D288Ef |
D288Er | 876-847 | GGTGCAGATCAGtTCAATTATCTTCCACTC | D288Ef |
D288Af | 847-876 | GAGTGGAAGATAATTGcTCTGATCTGCACC | D288A |
D288Ar | 876-847 | GGTGCAGATCAGAgCAATTATCTTCCACTC | D288A |
C291Sf | 855-885 | ATAATTGATCTGATCTcCACCCTAATCTTT | C291S |
C291Sr | 885-855 | AAAGATTAGGGTGgAGATCAGATCAATTAT | C291S |
C357Sf | 1053-1083 | AAGATTCTCTTGGCTTcTCATGTGAAAATC | C357S |
C357Sr | 1083-1053 | GATTTTCACATGAgAAGCCAAGAGAATCTT | C357S |